利用管家基因测序进行草绿色链球菌的菌种鉴定。
Use of housekeeping gene sequencing for species identification of viridans streptococci.
作者信息
Kiratisin Pattarachai, Li Li, Murray Patrick R, Fischer Steven H
机构信息
Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
出版信息
Diagn Microbiol Infect Dis. 2005 Apr;51(4):297-301. doi: 10.1016/j.diagmicrobio.2004.12.001.
Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes, zwf and gki, to identify members of the mitis-sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. This approach also suggested that some reference strains may not be correctly classified.
基于对20株参考菌株的基因分析，我们描述了一种方法，即通过对两个管家基因zwf和gki进行测序，将草绿色链球菌的缓症- sanguinis组成员鉴定到种水平，与16S rDNA测序相比，具有更好的鉴别能力。该方法还表明一些参考菌株可能分类不正确。
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