Martens Miet, Dawyndt Peter, Coopman Renata, Gillis Monique, De Vos Paul, Willems Anne
Laboratorium voor Microbiologie (WE10), Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):200-14. doi: 10.1099/ijs.0.65392-0.
There is a need for easy, practical, reliable and robust techniques for the identification and classification of bacterial isolates to the species level as alternatives to 16S rRNA gene sequence analysis and DNA-DNA hybridization. Here, we demonstrate that multilocus sequence analysis (MLSA) of housekeeping genes is a valuable alternative technique. An MLSA study of 10 housekeeping genes (atpD, dnaK, gap, glnA, gltA, gyrB, pnp, recA, rpoB and thrC) was performed on 34 representatives of the genus Ensifer. Genetic analysis and comparison with 16S and 23S rRNA gene sequences demonstrated clear species boundaries and a higher discrimination potential for all housekeeping genes. Comparison of housekeeping gene sequence data with DNA-DNA reassociation data revealed good correlation at the intraspecies level, but indicated that housekeeping gene sequencing is superior to DNA-DNA hybridization for the assessment of genetic relatedness between Ensifer species. Our MLSA data, confirmed by DNA-DNA hybridizations, support the suggestion that Ensifer xinjiangensis is a later heterotypic synonym of Ensifer fredii.
需要简便、实用、可靠且强大的技术，用于将细菌分离株鉴定和分类到种水平，作为16S rRNA基因序列分析和DNA-DNA杂交的替代方法。在此，我们证明管家基因的多位点序列分析（MLSA）是一种有价值的替代技术。对Ensifer属的34个代表菌株进行了10个管家基因（atpD、dnaK、gap、glnA、gltA、gyrB、pnp、recA、rpoB和thrC）的MLSA研究。遗传分析以及与16S和23S rRNA基因序列的比较表明，所有管家基因都有清晰的种界限和更高的鉴别潜力。管家基因序列数据与DNA-DNA重缔合数据的比较显示，在种内水平上有良好的相关性，但表明管家基因测序在评估Ensifer种间的遗传相关性方面优于DNA-DNA杂交。我们经DNA-DNA杂交证实的MLSA数据支持以下观点：新疆剑菌是费氏剑菌的晚出异名。
