Han Woong, Rhee Hae-Ik, Cho Jeong Woo, Ku Maurice S B, Song Pill Soon, Wang Myeong-Hyeon
Division of Biotechnology, Kangwon National University, Chuncheon, Kangwon-do 200-701, Republic of Korea.
Biochem Biophys Res Commun. 2005 May 13;330(3):887-90. doi: 10.1016/j.bbrc.2005.03.056.
Cyclin dependent kinases (CDKs) play important roles in the plant cell cycle, a highly coordinated process in plant growth and development. To understand the regulatory network involving the CDKs, we have examined the role of ACK1, a gene that has significant homology to known ICKs (inhibitors of CDKs), but occupies a distinct branch of the ICK phylogenetic tree. Overexpression of ACK1 in transgenic Arabidopsis significantly inhibited growth, leading to effects such as serration of leaves, as a result of strong inhibition of cell division in the leaf meristem. ACK1 transgenic plants also differed morphologically from control Arabidopsis plants, and the cells of ACK1 transgenics were more irregular than the corresponding cells of control plants. These results suggest that ACK1 acts as a CDK inhibitor in Arabidopsis, and that the alterations in leaf shape may be the result of restricted cell division.
细胞周期蛋白依赖性激酶（CDKs）在植物细胞周期中发挥重要作用，植物细胞周期是植物生长发育过程中一个高度协调的过程。为了了解涉及CDKs的调控网络，我们研究了ACK1的作用，ACK1是一个与已知的ICKs（CDKs抑制剂）具有显著同源性的基因，但位于ICK系统发育树的一个独特分支上。ACK1在转基因拟南芥中的过表达显著抑制了生长，导致叶片出现锯齿状等效应，这是由于叶片分生组织中的细胞分裂受到强烈抑制。ACK1转基因植物在形态上也与对照拟南芥植物不同，ACK1转基因植物的细胞比对照植物的相应细胞更不规则。这些结果表明，ACK1在拟南芥中作为CDK抑制剂发挥作用，叶片形状的改变可能是细胞分裂受限的结果。
