Tomiie Mari, Isaka Shigeyuki, Miyoshi Eiji, Taniguchi Naoyuki, Kimura Tadashi, Ogita Kazuhide, Tsutsui Tateki, Shimoya Koichiro, Nakagawa Takatoshi, Kondo Akihiro, Koyama Masayasu, Murata Yuji
Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita City, Osaka 565-0871, Japan.
Biochem Biophys Res Commun. 2005 May 13;330(3):999-1004. doi: 10.1016/j.bbrc.2005.02.186.
In early pregnancy, placental trophoblast cells rapidly grow and invade into maternal uterine tissue. N-Acetylglucosaminyltransferase V (GnT-V) and its product, beta1-6-GlcNAc branching glycan, are known to correlate with tumor invasion and metastasis. Since the placentation process resembles invasion of cancer cells, we examined the expression of beta1-6-GlcNAc branching glycan and GnT-V in human placenta. Placentas derived from the first trimester contained a larger amount of beta1-6-GlcNAc branching glycan, detected by leukoagglutinating phytohemagglutinin lectin blotting, than those at term. Immunohistochemical study revealed that beta1-6-GlcNAc branching glycans and GnT-V protein were localized in the trophoblast layer. Both protein expression and the enzyme activity of GnT-V in first trimester placentas were higher than those at term. These results suggest that GnT-V would contribute to placentation in the early phase of pregnancy, possibly regulating the process of invasion of trophoblast cells.
在妊娠早期，胎盘滋养层细胞迅速生长并侵入母体子宫组织。已知N-乙酰葡糖胺基转移酶V（GnT-V）及其产物β1-6-GlcNAc分支聚糖与肿瘤侵袭和转移相关。由于胎盘形成过程类似于癌细胞的侵袭，我们检测了β1-6-GlcNAc分支聚糖和GnT-V在人胎盘中的表达。通过白细胞凝集植物血凝素印迹法检测，孕早期胎盘所含的β1-6-GlcNAc分支聚糖比足月胎盘更多。免疫组织化学研究显示，β1-6-GlcNAc分支聚糖和GnT-V蛋白定位于滋养层。孕早期胎盘GnT-V的蛋白表达和酶活性均高于足月胎盘。这些结果表明，GnT-V可能在妊娠早期促进胎盘形成，可能调节滋养层细胞的侵袭过程。
