表达谱分析表明胆囊在脂质稳态中具有调节作用。

Expression profiling suggests a regulatory role of gallbladder in lipid homeostasis.

作者信息

Yuan Zuo-Biao, Han Tian-Quan, Jiang Zhao-Yan, Fei Jian, Zhang Yi, Qin Jian, Tian Zhi-Jie, Shang Jun, Jiang Zhi-Hong, Cai Xing-Xing, Jiang Yu, Zhang Sheng-Dao

机构信息

Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.

出版信息

World J Gastroenterol. 2005 Apr 14;11(14):2109-16. doi: 10.3748/wjg.v11.i14.2109.

DOI:10.3748/wjg.v11.i14.2109

PMID:15810076

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4305779/

Abstract

AIM

To examine expression profile of gallbladder using microarray and to investigate the role of gallbladder in lipid homeostasis.

METHODS

33P-labelled cDNA derived from total RNA of gallbladder tissue was hybridized to a cDNA array representing 17,000 cDNA clusters. Genes with intensities > or =2 and variation <0.33 between two samples were considered as positive signals with subtraction of background chosen from an area where no cDNA was spotted. The average gray level of two gallbladders was adopted to analyze its bioinformatics. Identified target genes were confirmed by touch-down polymerase chain reaction and sequencing.

RESULTS

A total of 11 047 genes expressed in normal gallbladder, which was more than that predicted by another author, and the first 10 genes highly expressed (high gray level in hybridization image), e.g. ARPC5 (2 225.88+/-90.46), LOC55972 (2 220.32+/-446.51) and SLC20A2 (1 865.21+/-98.02), were related to the function of smooth muscle contraction and material transport. Meanwhile, 149 lipid-related genes were expressed in the gallbladder, 89 of which were first identified (with gray level in hybridization image), e.g. FASN (11.42+/-2.62), APOD (92.61+/-8.90) and CYP21A2 (246.11+/-42.36), and they were involved in each step of lipid metabolism pathway. In addition, 19 of those 149 genes were gallstone candidate susceptibility genes (with gray level in hybridization image), e.g. HMGCR (10.98+/-0.31), NPC1 (34.88+/-12.12) and NR1H4 (16.8+/-0.65), which were previously thought to be expressed in the liver and/or intestine tissue only.

CONCLUSION

Gallbladder expresses 11 047 genes and takes part in lipid homeostasis.

摘要

目的

利用微阵列检测胆囊的基因表达谱，并研究胆囊在脂质稳态中的作用。

方法

将源自胆囊组织总RNA的33P标记的cDNA与代表17000个基因簇的cDNA阵列杂交。强度≥2且两个样本间变异<0.33的基因被视为阳性信号，背景扣除从无cDNA点样的区域选取。采用两个胆囊的平均灰度水平进行生物信息学分析。通过降落式聚合酶链反应和测序确认鉴定出的靶基因。

结果

正常胆囊中共表达11047个基因，比另一位作者预测的数量更多，前10个高表达基因（杂交图像中灰度值高），如ARPC5（2225.88±90.46）、LOC55972（2220.32±446.51）和SLC20A2（1865.21±98.02），与平滑肌收缩和物质转运功能相关。同时，胆囊中表达149个与脂质相关的基因，其中89个是首次鉴定（杂交图像中有灰度值），如FASN（11.42±2.62）、APOD（92.61±8.90）和CYP21A2（246.11±42.36），它们参与脂质代谢途径的各个步骤。此外，这149个基因中有19个是胆结石候选易感基因（杂交图像中有灰度值），如HMGCR（10.98±0.31）、NPC1（34.88±12.12）和NR1H4（16.8±0.65），这些基因以前被认为仅在肝脏和/或肠道组织中表达。