Luzhetskyy Andriy, Zhu Lili, Gibson Miranda, Fedoryshyn Marta, Dürr Clemens, Hofmann Carsten, Hoffmeister Dirk, Ostash Bohdan, Mattingly Cynthia, Adams Val, Fedorenko Victor, Rohr Jürgen, Bechthold Andreas
Institut für Pharmazeutische Wissenschaften, Albert-Ludwigs-Universität, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
Chembiochem. 2005 Apr;6(4):675-8. doi: 10.1002/cbic.200400316.
Two genes from Streptomyces cyanogenous S136 that encode the reductase LanZ4 and the hydroxylase LanZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis. Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O.
对产蓝链霉菌S136中参与地霉素A生物合成的两个基因进行了研究，这两个基因编码还原酶LanZ4和羟化酶LanZ5，采用了靶向基因失活的方法。对相应突变体的分析以及互补实验使我们能够证明，LanZ4和LanZ5负责地霉素生物合成过程中独特的C-11羟基化反应。lanZ4/Z5突变体积累的化合物是先前描述的地霉素F以及新的地霉素M和O。
