Menkes B, Prelipceanu O, Checiu I, Căpălnăşan I
Morphol Embryol (Bucur). 1979 Apr-Jun;25(2):119-26.
Besides the usual methods, vital fluorochroming may globally show the extent of the cytotoxic effect of Cyclophosphamide (CP). By intravital treatment with highly diluted fluorochromes (acridine-orange, etc.) necrobiotic and dead cells, cell debris and phagosomes appear electively fluorescent. Glycosaminoglycans and various mucopolysaccharides show a somewhat weaker fluorescence. Autopods and the mesencephalon were selected for comparative assessment of the effect of other cytotoxic agents. Important results were also obtained by the repeated examination of blood (erythrocytes) and of hemopoetic foci. Cytotoxic effect within the embryo, teratogenesis and chemotherapeutic action are strongly correlated.
除了常用方法外，活体荧光染色可全面显示环磷酰胺（CP）的细胞毒性作用程度。通过用高度稀释的荧光染料（吖啶橙等）进行活体处理，坏死和死亡细胞、细胞碎片和吞噬体呈现选择性荧光。糖胺聚糖和各种粘多糖显示出稍弱的荧光。选择足肢和中脑用于比较评估其他细胞毒性剂的作用。通过对血液（红细胞）和造血灶的反复检查也获得了重要结果。胚胎内的细胞毒性作用、致畸作用和化疗作用密切相关。
