Menkes B, Prelipceanu O, Checiu I, Căpălnăşan I
Morphol Embryol (Bucur). 1979 Oct-Dec;25(4):301-6.
The dynamics of the Cyclophosphamide (CPA) effect was investigated in the chick embryo by a vital fluorochroming method worked out previously. In the 4-day-old embryo the morphological changes (at the level of light microscopy) in the limbs and in the mesencephalon induced by 100 microgram CPA appeared after a period of latency of about 10 hours. Successive blood control in the same embryo, treated with 50 microgram CPA, revealed three consecutive modifications of the blood cells: a great number of abnormal mitoses, the appearance of a great number of multinucleated, nonviable cells and the recovery of the lost cells. No difference could be detected between the effect of freshly prepared and stored CPA solutions. The fate of CPA (a bifunctionally alkylating agent) in contact with living tissues is discussed.
采用先前建立的活体细胞荧光染色法，研究了环磷酰胺（CPA）对鸡胚的作用动力学。在4日龄鸡胚中，100微克CPA诱导的四肢和中脑形态学变化（在光学显微镜水平）在约10小时的潜伏期后出现。用50微克CPA处理同一胚胎后连续进行血液检查，发现血细胞有三种连续变化：大量异常有丝分裂、大量多核非存活细胞的出现以及丢失细胞的恢复。新鲜配制的和储存的CPA溶液的作用未检测到差异。讨论了双功能烷化剂CPA与活组织接触后的转归。
