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柠檬致病真菌和非致病真菌的分离、鉴定及抗真菌药敏试验

Isolation, identification and antifungal susceptibility of lemon pathogenic and non pathogenic fungi.

作者信息

Maldonado María Cristina, Santa Runco Rosa, Navarro Antonio Roberto

机构信息

Instituto de Biotecnología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina.

出版信息

Rev Iberoam Micol. 2005 Mar;22(1):57-9. doi: 10.1016/s1130-1406(05)70009-7.

Abstract

Numerous species of filamentous fungi were isolated from lemon on different plantations in the province of Tucuman, Argentina. The techniques suggested by the Subcommittee of Antifungal Susceptibility of the National Committee for Clinical Laboratory Standards, (USA) were adapted. The effect of three different concentrations of the fungicides imazalil, guazatine, SOPP and thiabendazole on the fungi Fusarium oxysporum, Fusarium moniliforme, Aspergillus niger, Aspergillus flavus, Aspergillus clavatus, Geotrichum candidum, Rhizopus sp, Penicillium sp, Penicillium digitatum and Mucor sp were studied. All the tested strains were resistant to thiabendazole. We assayed a mixture of SOPP (5%), guazatine (350 ppm) and imazalil (100 ppm), which showed a synergic effect on Rhizopus sp. Mucor sp was the only fungus resistant to the four fungicides tested as well as to the above mentioned mixture.

摘要

在阿根廷图库曼省的不同种植园里,从柠檬中分离出了许多丝状真菌物种。采用了美国国家临床实验室标准委员会抗真菌药敏小组委员会建议的技术。研究了三种不同浓度的杀菌剂抑霉唑、双胍辛胺、邻苯基苯酚(SOPP)和噻菌灵对尖孢镰刀菌、串珠镰刀菌、黑曲霉、黄曲霉、棒曲霉、白地霉、根霉属、青霉属、指状青霉和毛霉属真菌的影响。所有测试菌株对噻菌灵均有抗性。我们检测了一种由5%的SOPP、350 ppm的双胍辛胺和100 ppm的抑霉唑组成的混合物,该混合物对根霉属真菌显示出协同作用。毛霉属是唯一对所测试的四种杀菌剂以及上述混合物都具有抗性的真菌。

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