Zorn-Kruppa Michaela, Tykhonova Svitlana, Belge Gazanfer, Bednarz Jürgen, Diehl Horst A, Engelke Maria
Institute of Biophysics, University of Bremen, Otto Hahn Allee 1, 28359 Bremen, Germany.
Altern Lab Anim. 2005 Feb;33(1):37-45. doi: 10.1177/026119290503300107.
Within the last decade, extensive research in the field of tissue and organ engineering has focused on the development of in vitro models of the cornea. The use of organotypic, three-dimensional corneal equivalents has several advantages over simple monolayer cultures. The aim of this study was to develop a corneal equivalent model composed of the same cell types as in the natural human tissue, but by using immortalised cell lines to ensure reproducibility and to minimise product variation. We report our success in the establishment of an SV40-immortalised human corneal keratocyte cell line (designated HCK). A collagen matrix, built up with these cells, displayed the morphological characteristics of the human stromal tissue and served as a biomatrix for the immortalised human corneal epithelial and endothelial cells. Histological cross-sections of the whole-cornea equivalents resemble human corneas in tissue structure. This organotypic in vitro model may serve as a research tool for the ophthalmic science community, as well as a model system for testing for eye irritancy and drug efficacy.
在过去十年中，组织和器官工程领域的广泛研究聚焦于角膜体外模型的开发。与简单的单层培养相比，使用器官型三维角膜等效物具有多个优势。本研究的目的是开发一种由与天然人体组织相同细胞类型组成的角膜等效模型，但使用永生化细胞系以确保可重复性并最小化产品差异。我们报告了成功建立SV40永生化人角膜基质细胞系（命名为HCK）。用这些细胞构建的胶原基质展现出人类基质组织的形态特征，并作为永生化人角膜上皮细胞和内皮细胞的生物基质。全角膜等效物的组织学横截面在组织结构上类似于人类角膜。这种器官型体外模型可作为眼科科学界的研究工具，以及用于测试眼刺激性和药物疗效的模型系统。
