Zorn-Kruppa Michaela, Tykhonova Svitlana, Belge Gazanfer, Diehl Horst A, Engelke Maria
Institut für Biophysik, Universität Bremen, D-28359 Bremen, Germany.
ALTEX. 2004;21(3):129-34.
The cytotoxic pattern of cosmetic or pharmaceutical compounds within different layers of the human cornea is of special interest with respect to ocular safety testing. The aim of this study was to evaluate the ability of a newly developed human corneal keratocyte (HCK) cell line as an in vitro model to predict toxicity towards keratocytes in the corneal stroma. The cytotoxic response of immortalised HCK cultures towards different surfactants was compared to that of primary cultures of human corneal keratocytes. Our studies revealed comparable results for immortalised and primary keratocytes. Furthermore, we quantified surfactant-induced cytotoxic effects on immortalised cultures of corneal epithelium and endothelium. In conclusion, the HCK cell line represents an appropriate model to test keratocyte-specific toxicity and may serve as a useful building block in the construction of three-dimensional human cornea equivalent models.
就眼部安全性测试而言，化妆品或药物化合物在人角膜不同层中的细胞毒性模式具有特殊意义。本研究的目的是评估一种新开发的人角膜基质细胞（HCK）细胞系作为体外模型预测对角膜基质中角膜基质细胞毒性的能力。将永生化HCK培养物对不同表面活性剂的细胞毒性反应与人角膜基质细胞原代培养物的细胞毒性反应进行比较。我们的研究揭示了永生化和原代角膜基质细胞的可比结果。此外，我们量化了表面活性剂对角膜上皮和内皮永生化培养物的细胞毒性作用。总之，HCK细胞系是测试角膜基质细胞特异性毒性的合适模型，可作为构建三维人角膜等效模型的有用组成部分。
