唇癌中肥大细胞亚群的特征分析
Characterization of mast cell subpopulations in lip cancer.
作者信息
Rojas I G, Spencer M L, Martínez A, Maurelia M A, Rudolph M I
机构信息
Department of Oral Surgery, College of Dentistry, Universidad de Concepción, Casilla 160 C, Concepcion, Chile.
出版信息
J Oral Pathol Med. 2005 May;34(5):268-73. doi: 10.1111/j.1600-0714.2004.00297.x.
BACKGROUND
Lip squamous cell carcinoma (SCC) is the most common form of oral cancer. Human mast cells (MCs), which are increased in lip SCC, are classified by their protease content in tryptase-positive (MC(T)) and tryptase/chymase-positive (MC(TC)). MC proteases are associated with tumor progression and angiogenesis. The aim of this study was to quantify and characterize MC subpopulations in lip SCC.
METHODS
Serial sections from lip SCC (n = 21) and normal lip vermilion (n = 8) biopsies were stained immunohistochemically for tryptase and enzymehistochemically for chymase to determine MC subpopulation density and distribution.
RESULTS
MC(T) and MC(TC) were increased in lip SCC when compared with normal lip (P < 0.0001), where MC(T) predominated over MC(TC) (P < 0.01). In lip SCC neither subpopulation predominated. Regarding distribution, MC(T) were higher than MC(TC) at the intratumoral stroma, whereas MC(TC) were higher than MC(T) at the peritumoral stroma (P < 0.01).
CONCLUSIONS
The results suggest that MC subpopulations may contribute to lip SCC progression. While intratumoral MC(T) may stimulate angiogenesis, peritumoral MC(TC) may promote extracellular matrix degradation and tumor progression at the invasion front.
背景
唇鳞状细胞癌（SCC）是口腔癌最常见的形式。人类肥大细胞（MCs）在唇SCC中数量增加，根据其蛋白酶含量可分为类胰蛋白酶阳性（MC(T)）和类胰蛋白酶/糜蛋白酶阳性（MC(TC)）。MC蛋白酶与肿瘤进展和血管生成有关。本研究的目的是对唇SCC中的MC亚群进行定量和表征。
方法
对唇SCC（n = 21）和正常唇红（n = 8）活检组织的连续切片进行免疫组织化学染色检测类胰蛋白酶，酶组织化学染色检测糜蛋白酶，以确定MC亚群的密度和分布。
结果
与正常唇相比，唇SCC中的MC(T)和MC(TC)增加（P < 0.0001），其中MC(T)多于MC(TC)（P < 0.01）。在唇SCC中，两个亚群均不占优势。关于分布，肿瘤内基质中的MC(T)高于MC(TC)，而肿瘤周围基质中的MC(TC)高于MC(T)（P < 0.01）。
结论
结果表明MC亚群可能有助于唇SCC的进展。肿瘤内的MC(T)可能刺激血管生成，而肿瘤周围的MC(TC)可能促进细胞外基质降解和肿瘤在侵袭前沿的进展。
Zotero 插件