Atarbashi-Moghadam Saede, Niazmand Maral, Vafadar Shahla, Gholami Toghchi Sanaz
Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
General Dentist, Research Center, Dental School Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Dent (Shiraz). 2024 Dec 1;25(4):369-373. doi: 10.30476/dentjods.2024.99652.2164. eCollection 2024 Dec.
Squamous cell carcinomas (SCCs) and premalignant disorders such as leukoplakia are common oral cavity lesions. Although these lesions are epithelial in nature, they are also associated with juxta-epithelial chronic inflammation. Mast cells play a significant role in inflammation initiation and propagation.
Previous studies have yielded conflicting results in this field. Therefore, this research aimed to assess the number of mast cells in oral SCC and dysplastic leukoplakia and explore their possible role in these lesions.
In this retrospective cross-sectional study, sixty-three archival cases, including 22 OSCCs, 28 dysplastic leukoplakias as epithelial dysplasia (ED), and 13 normal oral mucosal tissues, were examined for mast cells, using toluidine blue staining. Hotspot areas were identified at 10× magnification and mast cells were counted in 5 fields at 40× magnification. The average cell numbers were calculated, and the severity of inflammation was scored. Statistical analysis was performed using SPSS statistical software 20, including One-way ANOVA, Two-way ANOVA, paired-t test, and independent t-test. Value < 0.05 was considered significant.
Among the 51 pathologic lesions, 54.9% were males and 45.1% were females, with a mean age of 56.34±15.35 years. The most common locations were the tongue and buccal mucosa. The mast cell count was significantly lower in SCC compared to ED (= 0.009). There was no correlation between mast cell count and inflammation score (= 0.345).
In this study, the mast cell count was higher in ED compared to OSCC, suggesting an increase in these cells during the pre-malignant stages. However, the number of mast cells decreased after connective tissue invasion and microenvironmental changes occurred.
鳞状细胞癌（SCC）和癌前病变如白斑是常见的口腔病变。尽管这些病变本质上是上皮性的，但它们也与上皮下慢性炎症相关。肥大细胞在炎症的起始和传播中起重要作用。
先前的研究在该领域得出了相互矛盾的结果。因此，本研究旨在评估口腔鳞状细胞癌和发育异常性白斑中肥大细胞的数量，并探讨它们在这些病变中的可能作用。
在这项回顾性横断面研究中，使用甲苯胺蓝染色对63例存档病例进行肥大细胞检查，其中包括22例口腔鳞状细胞癌、28例作为上皮发育异常（ED）的发育异常性白斑和13例正常口腔黏膜组织。在10倍放大倍数下确定热点区域，并在40倍放大倍数下在5个视野中对肥大细胞进行计数。计算平均细胞数，并对炎症严重程度进行评分。使用SPSS统计软件20进行统计分析，包括单因素方差分析、双因素方差分析、配对t检验和独立t检验。P值<0.05被认为具有统计学意义。
在51例病理病变中，男性占54.9%，女性占45.1%，平均年龄为56.34±15.35岁。最常见的部位是舌和颊黏膜。与ED相比，SCC中的肥大细胞计数显著降低（P = 0.009）。肥大细胞计数与炎症评分之间无相关性（P = 0.345）。
在本研究中，与口腔鳞状细胞癌相比，ED中的肥大细胞计数更高，表明在癌前阶段这些细胞数量增加。然而，在结缔组织浸润和微环境变化发生后，肥大细胞数量减少。
