Rublee Parke A, Remington David L, Schaefer Eric F, Marshall Michael M
Biology Department, University of North Carolina at Greensboro, PO Box 26170, Greensboro, North Carolina 27402-6170, USA.
J Eukaryot Microbiol. 2005 Mar-Apr;52(2):83-9. doi: 10.1111/j.1550-7408.2005.05202007.x.
Molecular methods, including conventional PCR, real-time PCR, denaturing gradient gel electrophoresis, fluorescent fragment detection PCR, and fluorescent in situ hybridization, have all been developed for use in identifying and studying the distribution of the toxic dinoflagellates Pfiesteria piscicida and P. shumwayae. Application of the methods has demonstrated a worldwide distribution of both species and provided insight into their environmental tolerance range and temporal changes in distribution. Genetic variability among geographic locations generally appears low in rDNA genes, and detection of the organisms in ballast water is consistent with rapid dispersal or high gene flow among populations, but additional sequence data are needed to verify this hypothesis. The rapid development and application of these tools serves as a model for study of other microbial taxa and provides a basis for future development of tools that can simultaneously detect multiple targets.
分子方法，包括传统PCR、实时PCR、变性梯度凝胶电泳、荧光片段检测PCR和荧光原位杂交，都已被开发用于鉴定和研究有毒甲藻——杀鱼费氏藻（Pfiesteria piscicida）和舒氏费氏藻（P. shumwayae）的分布。这些方法的应用已证明这两个物种在全球范围内均有分布，并深入了解了它们的环境耐受范围和分布的时间变化。rDNA基因中不同地理位置之间的遗传变异性通常较低，在压舱水中检测到这些生物与种群之间的快速扩散或高基因流一致，但需要更多的序列数据来验证这一假设。这些工具的快速发展和应用为研究其他微生物类群提供了一个模型，并为未来同时检测多个目标的工具开发奠定了基础。
