Oikonomou Andreas, Katsiapi Matina, Karayanni Hera, Moustaka-Gouni Maria, Kormas Konstantinos Ar
Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, 384 46 Volos, Greece.
ScientificWorldJournal. 2012;2012:504135. doi: 10.1100/2012/504135. Epub 2012 May 1.
Lake Karla, Greece, was dried up in 1962 and its refilling started in 2009. We examined the Cyanobacteria and unicellular eukaryotes found during two fish kill incidents, in March and April 2010, in order to detect possible causative agents. Both microscopic and molecular (16S/18S rRNA gene diversity) identification were applied. Potentially toxic Cyanobacteria included representatives of the Planktothrix and Anabaena groups. Known toxic eukaryotes or parasites related to fish kill events were Prymnesium parvum and Pfiesteria cf. piscicida, the latter being reported in an inland lake for the second time. Other potentially harmful microorganisms, for fish and other aquatic life, included representatives of Fungi, Mesomycetozoa, Alveolata, and Heterokontophyta (stramenopiles). In addition, Euglenophyta, Chlorophyta, and diatoms were represented by species indicative of hypertrophic conditions. The pioneers of L. Karla's plankton during the first months of its water refilling process included species that could cause the two observed fish kill events.
希腊的卡拉湖于1962年干涸，2009年开始重新蓄水。我们对2010年3月和4月两次鱼类死亡事件中发现的蓝藻和单细胞真核生物进行了检测，以找出可能的致病因子。我们采用了显微镜鉴定和分子（16S/18S rRNA基因多样性）鉴定方法。具有潜在毒性的蓝藻包括席藻属和鱼腥藻属的代表物种。与鱼类死亡事件相关的已知有毒真核生物或寄生虫有微小原甲藻和类杀鱼费氏藻，后者是在内陆湖泊中的第二次报道。其他对鱼类和其他水生生物有潜在危害的微生物包括真菌、中黏菌门、囊泡虫类和不等鞭毛类（硅藻）的代表物种。此外，裸藻门、绿藻门和硅藻由指示富营养化状况的物种代表。在卡拉湖重新蓄水过程的头几个月中，其浮游生物的先锋物种包括可能导致两次观察到的鱼类死亡事件的物种。
