Jobin Guy, Grondin Gilles, Couture Geneviève, Beaulieu Carole
Centre d'Etude et de Valorisation de la Diversité Microbienne, Département de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500, boul. Universitété Sherbrooke, Québec J1K 2R1, Canada.
Microsc Microanal. 2005 Apr;11(2):154-65. doi: 10.1017/S1431927605050142.
Spores of the biocontrol agent, Streptomyces melanosporofaciens EF-76, were entrapped by complex coacervation in beads composed of a macromolecular complex (MC) of chitosan and polyphosphate. A proportion of spores entrapped in beads survived the entrapment procedure as shown by treating spores from chitosan beads with a dye allowing the differentiation of live and dead cells. The spore-loaded chitosan beads could be digested by a chitosanase, suggesting that, once introduced in soil, the beads would be degraded to release the biocontrol agent. Spore-loaded beads were examined by optical and scanning electron microscopy because the release of the biological agent depends on the spore distribution in the chitosan beads. The microscopic examination revealed that the beads had a porous surface and contained a network of inner microfibrils. Spores were entrapped in both the chitosan microfibrils and the bead lacuna.
生防菌黑色素产孢链霉菌（Streptomyces melanosporofaciens）EF-76的孢子通过复凝聚法包裹于由壳聚糖和聚磷酸盐的大分子复合物（MC）组成的珠粒中。用一种可区分活细胞和死细胞的染料处理壳聚糖珠粒中的孢子，结果表明，包裹于珠粒中的一部分孢子在包裹过程中存活了下来。负载孢子的壳聚糖珠粒可被壳聚糖酶消化，这表明一旦引入土壤，珠粒将会降解以释放生防菌。由于生物制剂的释放取决于壳聚糖珠粒中孢子的分布情况，因此对负载孢子的珠粒进行了光学显微镜和扫描电子显微镜检查。显微镜检查显示，珠粒表面具有多孔性，且内部含有微纤丝网络。孢子既包裹于壳聚糖微纤丝中，也存在于珠粒腔隙中。
