Laboratório de Ecologia Microbiana e Biotecnologia (LEMBiotech), Departamento de Biologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
Bioresour Technol. 2010 Apr;101(7):2121-5. doi: 10.1016/j.biortech.2009.11.004. Epub 2009 Nov 27.
Spores of Bacillus subtilis LAMI008 were entrapped in 3-mm chitosan beads and cross-linked with 0.3% glutaraldehyde for n-hexadecane biodegradation and biosurfactant recovery. When exposed to nutrients, the spores generated vegetative cells without morphological alterations as revealed by atomic force microscopy. The entrapped cells degraded almost 100% of 1% of n-hexadecane in medium supplemented with 1% glucose and produce biosurfactant within 48 h, as well as free cells. The number of viable cells inside the beads was maintained throughout the n-hexadecane degradation process and the released biosurfactant was not used as a carbon source. Entrapment of bacterial spores in chitosan beads overcomes problems with stability, storage, and long term cell viability encountered with vegetative cells. This approach can potentially be utilized for biodegradation of complex compounds by entrapping spores of different species of bacteria.
枯草芽孢杆菌 LAMI008 的孢子被包埋在 3 毫米大小的壳聚糖珠中，并与 0.3%戊二醛交联，用于正十六烷的生物降解和生物表面活性剂的回收。当暴露于营养物质时，孢子产生了营养细胞，而原子力显微镜显示这些细胞没有形态上的改变。包埋细胞在补充有 1%葡萄糖的培养基中几乎降解了 1%的正十六烷，并在 48 小时内产生了生物表面活性剂，与游离细胞一样。在正十六烷降解过程中，珠内的活细胞数量保持不变，释放的生物表面活性剂也未被用作碳源。将细菌孢子包埋在壳聚糖珠中克服了营养细胞在稳定性、储存和长期细胞活力方面遇到的问题。这种方法可以通过包埋不同种细菌的孢子，用于复杂化合物的生物降解。
