Welsh David K, Imaizumi Takato, Kay Steve A
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Methods Enzymol. 2005;393:269-88. doi: 10.1016/S0076-6879(05)93011-5.
Luciferase enzymes have been used as reporters of circadian rhythms in organisms as diverse as cyanobacteria, plants, fruit flies, and mice. This article details methodology for real-time reporting of circadian-regulated gene expression by imaging of luciferase bioluminescence in plants and mammalian cells.
荧光素酶已被用作多种生物（如蓝细菌、植物、果蝇和小鼠）昼夜节律的报告基因。本文详细介绍了通过对植物和哺乳动物细胞中荧光素酶生物发光进行成像来实时报告昼夜节律调节基因表达的方法。
