Walker John R, Hogenesch John B
The Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA.
Methods Enzymol. 2005;393:366-76. doi: 10.1016/S0076-6879(05)93016-4.
DNA arrays have become indispensable tools in the study of transcriptional output of the circadian clock and have enabled an increase in the number of outputs from tens to thousands. However, despite their widespread use, many challenges exist in accurately and sensitively identifying all transcriptional targets of the clock. This article discusses aspects of experimental design, including RNA-amplification strategies, data condensation methods, and other aspects that impact the use of these tools for the study of circadian biology.
DNA阵列已成为研究生物钟转录输出不可或缺的工具，使得输出数量从几十种增加到数千种。然而，尽管它们被广泛使用，但在准确且灵敏地识别生物钟的所有转录靶点方面仍存在许多挑战。本文讨论了实验设计的各个方面，包括RNA扩增策略、数据浓缩方法以及其他影响这些工具用于昼夜节律生物学研究的方面。
