Fan Jian-Gao, Zhong Lan, Tia Li-Yan, Xu Zheng-Jie, Li Min-Sheng, Wang Guo-Liang
Department of Gastroenterology, Shanghai First People's Hospital, Shanghai 200080, China.
World J Gastroenterol. 2005 Apr 21;11(15):2346-50. doi: 10.3748/wjg.v11.i15.2346.
To evaluate the effects of ursodeoxycholic acid (UDCA) and/or low-calorie diet (LCD) on a rat model of nonalcoholic steatohepatitis (NASH).
Fifty-five Sprague-Dawley rats were divided into five groups. The control group (n = 9) was fed with standard rat diet for 12 wk, NASH group (n = 10) was fed with high-fat diet consisted of normal diet, 10% lard oil and 2% cholesterol for 12 wk, UDCA group (n = 10) was fed with high-fat diet supplemented with UDCA at a dose of 25 mg/(kg.d) in drinking water for 12 wk, LCD group (n = 10) was fed with high-fat diet for 10 wk and then LCD for 2 wk, and UDCA+LCD group (n = 15) was fed with high-fat diet for 10 wk, followed by LCD+UDCA for 2 wk. At the end of the experiment, body weight, serum biochemical index, and hepatopathologic changes were examined.
Compared with the control group, rats in the NASH group had significantly increased body weight, liver weight, and serum lipid and aminotransferase levels. All rats in the NASH group developed steatohepatitis, as determined by their liver histology. Compared with the NASH group, there were no significant changes in body weight, liver weight, blood biochemical index, the degree of hepatic steatosis, and histological activity index (HAI) score in the UDCA group; however, body and liver weights were significantly decreased, and the degree of steatosis was markedly improved in rats of both the LCD group and the UDCA+LCD group, but significant improvement with regard to serum lipid variables and hepatic inflammatory changes were seen only in rats of the UDCA+LCD group, and not in the LCD group.
LCD might play a role in the treatment of obesity and hepatic steatosis in rats, but it exerts no significant effect on both serum lipid disorders and hepatic inflammatory changes. UDCA may enhance the therapeutic effects of LCD on steatohepatitis accompanied by obesity and hyperlipidemia. However, UDCA alone is not effective in the prevention of steatohepatitis induced by high-fat diet.
评估熊去氧胆酸（UDCA）和/或低热量饮食（LCD）对非酒精性脂肪性肝炎（NASH）大鼠模型的影响。
将55只Sprague-Dawley大鼠分为五组。对照组（n = 9）给予标准大鼠饮食12周，NASH组（n = 10）给予由普通饮食、10%猪油和2%胆固醇组成的高脂饮食12周，UDCA组（n = 10）给予高脂饮食并在饮水中补充剂量为25 mg/(kg·d)的UDCA，持续12周，LCD组（n = 10）给予高脂饮食10周，然后给予LCD 2周，UDCA + LCD组（n = 15）给予高脂饮食10周，随后给予LCD + UDCA 2周。实验结束时，检测体重、血清生化指标和肝脏病理变化。
与对照组相比，NASH组大鼠体重、肝脏重量以及血脂和转氨酶水平显著升高。根据肝脏组织学检查，NASH组所有大鼠均发生脂肪性肝炎。与NASH组相比，UDCA组大鼠体重、肝脏重量以及血液生化指标、肝脂肪变性程度和组织学活动指数（HAI）评分均无显著变化；然而，LCD组和UDCA + LCD组大鼠的体重和肝脏重量均显著降低，脂肪变性程度明显改善，但仅UDCA + LCD组大鼠的血脂变量和肝脏炎症变化有显著改善，LCD组则无。
LCD可能对大鼠肥胖和肝脂肪变性有治疗作用，但对血脂紊乱和肝脏炎症变化无显著影响。UDCA可增强LCD对伴有肥胖和高脂血症的脂肪性肝炎的治疗效果。然而，单独使用UDCA对预防高脂饮食诱导的脂肪性肝炎无效。
