Barrios L, Miró R, Corominas M, Pellicer A, Egozcue J
Departament de Biologia Cellular i Fisiologia Universitat Autonoma de Barcelona, Spain.
Cancer Genet Cytogenet. 1992 Apr;59(2):217-8. doi: 10.1016/0165-4608(92)90219-x.
We describe a simple method for isolation of high-molecular-weight DNA from cells fixed in Carnoy's solution for cytogenetic studies. DNA samples were extracted from NIH3T3 cells, and no apparent degradation was noticed. This method should be useful for molecular analysis of cells fixed for cytogenetic studies.
我们描述了一种从用卡诺氏液固定用于细胞遗传学研究的细胞中分离高分子量DNA的简单方法。从NIH3T3细胞中提取DNA样本，未发现明显降解。该方法对于固定用于细胞遗传学研究的细胞的分子分析应是有用的。
