Pereira Marina A, Dias Andre R, Faraj Sheila F, Cirqueira Cinthya dos S, Tomitao Michele T, Nahas Sergio Carlos, Ribeiro Ulysses, de Mello Evandro S
Cancer Institute(ICESP), University of Sao Paulo School of Medicine, Sao Paulo, Brazil.
Histopathology. 2015 Feb;66(3):388-97. doi: 10.1111/his.12532. Epub 2014 Nov 10.
To compare Carnoy's solution (CS) and 10% neutral buffered formalin solution (NBF) as tissue fixatives in colorectal cancer specimens.
Surgical specimens from patients with colorectal cancer were analysed. Three groups were studied, as follows: group 1 consisted of 16 paired samples fixed in CS and NBF; and groups 2 and 3 consisted of 14 prospective and 80 retrospective samples, respectively, both randomized for fixation in CS or NBF. Groups 1 and 2 were analysed for amount, quality and integrity of DNA. Morphological analysis, including some of the usual special stains and polymerase chain reaction (PCR), were also performed for group 1, and Sanger sequencing for group 2. Immunohistochemical (IHC) reactions for mismatch repair proteins were studied in groups 1 and 3. Fixative performances were similar for morphology, special stains, and IHC reactions, as well as for the amount, quality and integrity of extracted DNA. PCR amplification was not possible in two cases from CS group 1. Sanger sequencing gave conclusive results for the CS samples tested.
Carnoy's solution and NBF are equivalent fixatives for colorectal cancer specimens and are adequate for routine utilization in surgical and molecular pathology.
比较卡诺氏固定液（CS）和10%中性缓冲福尔马林固定液（NBF）在结直肠癌标本中作为组织固定剂的效果。
对结直肠癌患者的手术标本进行分析。研究分为三组，如下：第1组由16对分别用CS和NBF固定的样本组成；第2组和第3组分别由14例前瞻性样本和80例回顾性样本组成，两组样本均随机用CS或NBF固定。对第1组和第2组样本的DNA量、质量和完整性进行分析。对第1组样本进行形态学分析，包括一些常用的特殊染色和聚合酶链反应（PCR），对第2组样本进行桑格测序。对第1组和第3组样本研究错配修复蛋白的免疫组织化学（IHC）反应。在形态学、特殊染色、IHC反应以及提取DNA的量、质量和完整性方面，两种固定剂的效果相似。第1组CS组的两个样本无法进行PCR扩增。对所检测的CS样本，桑格测序给出了明确结果。
卡诺氏固定液和NBF作为结直肠癌标本的固定剂效果相当，适用于外科和分子病理学的常规应用。
