Shoemaker R H, Smythe A M, Wu L, Balaschak M S, Boyd M R
Laboratory of Drug Discovery Research and Development, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702.
Cancer Res. 1992 May 15;52(10):2791-6.
A sensitive DNA dot-blot assay for repetitive human DNA sequences was developed and applied to the quantitative determination of spontaneous metastases of a human melanoma in various tissues of nude mice. The assay was useful for defining the time course and pattern of tissue distribution of metastatic cells as well as for assessing response to therapy. The methodology is relatively simple, can be performed using nonradioactive DNA probes, and should be broadly applicable to studies of metastasis of human tumors in nude mice.
我们开发了一种用于检测人类重复DNA序列的灵敏DNA斑点印迹法，并将其应用于定量测定人类黑色素瘤在裸鼠各种组织中的自发转移情况。该检测方法有助于确定转移细胞的时间进程和组织分布模式，以及评估对治疗的反应。该方法相对简单，可以使用非放射性DNA探针进行操作，并且应该广泛适用于裸鼠体内人类肿瘤转移的研究。
