Liu C, Zhao H, Jin L
Department of Chemistry, Beijing Normal University, 100875 Beijing.
Guang Pu Xue Yu Guang Pu Fen Xi. 1999 Jun;19(3):447-9.
Intramolecular energy transfer occurs in the terbium complex with pipemidic acid (PPA) and the terbium(III) ion fluorescence can be sensitized by PPA. The fluorescence intensity of the Tb3+-PPA system is proportional to the amount of PPA. Accordingly, a fluorimetric method for the determination of PPA is proposed. The characteristics of the method include low background noise, high selectivity and long fluorescence lifetime. The method can be applied to the determination of PPA in urine and serum. The recovery is in the range of 94.6%-102%. Its detection limit and linear range for PPA are 3.6 ng/mL and 5.0 x 10(-8)-4.0 x 10(-6) mol/L, respectively.
在与吡哌酸（PPA）形成的铽配合物中发生分子内能量转移，并且PPA可使铽（III）离子荧光增强。Tb3 + -PPA体系的荧光强度与PPA的量成正比。因此，提出了一种测定PPA的荧光分析法。该方法的特点包括背景噪声低、选择性高和荧光寿命长。该方法可用于尿液和血清中PPA的测定。回收率在94.6% - 102%范围内。其对PPA的检测限和线性范围分别为3.6 ng/mL和5.0×10(-8)-4.0×10(-6) mol/L。
