Zhao Chun-gui, Li Xiao-li, Yang Bin-sheng
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2007 Dec;27(12):2546-9.
The fluorescence of terbium was sensitized after addition of terbium to the ethylene-N, N'-bis (o-hydioxyphenylglycine) (EHPG) solution. A novel and simple method used for the determination of Tb (III) was developed by means of fluorescence spectroscopy in the presence of EHPG. It was showed that the relative fluorescence intensity is proportional to the concentration of terbium ions, while the molar ratio of terbium to EHPG is less than 1.0 in the system. The maximum wavelengths of excitation and emission are 295 and 547 nm respectively. The optimal range of pH is 7-9. The linear range of detection of the concentration of terbium is from 1.0 x 10(-8) mol x L(-1) to 1.0 x 10(-5) mol x L(-1), with a detection limit of 1.18 x 10(-9) mol x L(-1). The relative standard deviation is still within +/-3% in the presence of other lanthanide ions. The method was applied to the determination of the recoveries of synthetic samples and a rare earth sample with satisfactory results.
向乙烯 - N,N'-双（邻羟基苯基甘氨酸）（EHPG）溶液中加入铽后，铽的荧光被敏化。在EHPG存在下，通过荧光光谱法开发了一种用于测定铽（III）的新颖且简单的方法。结果表明，在该体系中铽与EHPG的摩尔比小于1.0时，相对荧光强度与铽离子浓度成正比。激发和发射的最大波长分别为295和547nm。最佳pH范围为7 - 9。铽浓度的检测线性范围为1.0×10⁻⁸mol·L⁻¹至1.0×10⁻⁵mol·L⁻¹，检测限为1.18×10⁻⁹mol·L⁻¹。在存在其他镧系离子的情况下，相对标准偏差仍在±3%以内。该方法应用于合成样品和稀土样品回收率的测定，结果令人满意。
