Ferrari G, De Liberato C, Scavia G, Lorenzetti R, Zini M, Farina F, Magliano A, Cardeti G, Scholl F, Guidoni M, Scicluna M T, Amaddeo D, Scaramozzino P, Autorino G L
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Via Appia Nuova 1411, 00178 Rome, Italy.
Prev Vet Med. 2005 May 10;68(2-4):103-13. doi: 10.1016/j.prevetmed.2004.11.011. Epub 2005 Jan 18.
Several seroconversions occurring in 2002 among sentinel cattle during the bluetongue-vaccination campaign in Lazio and Tuscany (central Italy) led to the suspicion of vaccine-virus circulation. Therefore in 2003, 17 seroconverting sentinel herds were investigated for the characteristics of the virus involved. From these farms, 91 unvaccinated animals and 57 Culicoides pools were tested for the presence of the bluetongue vaccine virus (serotype-2) or other strains. The presence of vaccine virus serotype-2 was confirmed by PCR followed by restriction analysis in the whole blood of 17 unvaccinated sentinel cattle and 12 pools of Culicoides imicola or C. obsoletus. Of the 17 herds, five were positive only for vaccine virus serotype-2, four were positive for other strains and two for both the vaccine and other strains; the remaining premises were virologicaly negative. The vaccine virus serotype-2 also was detected in areas not included in the vaccination campaign.
2002年，在意大利中部拉齐奥和托斯卡纳开展的蓝舌病疫苗接种运动期间，哨兵牛群中出现了几起血清转化事件，这引发了对疫苗病毒传播的怀疑。因此，2003年，对17个血清转化的哨兵牛群进行了调查，以了解所涉病毒的特征。从这些农场中，对91头未接种疫苗的动物和57组库蠓进行了检测，以确定是否存在蓝舌病疫苗病毒（血清型2）或其他毒株。通过PCR以及对17头未接种疫苗的哨兵牛和12组小马库蠓或废弃库蠓的全血进行限制性分析，证实了血清型2疫苗病毒的存在。在这17个牛群中，5个仅对血清型2疫苗病毒呈阳性，4个对其他毒株呈阳性，2个对疫苗和其他毒株均呈阳性；其余养殖场在病毒学检测中呈阴性。在疫苗接种运动未覆盖的地区也检测到了血清型2疫苗病毒。
