Gorch C, Vagnozzi A, Duffy S, Miquet J, Pacheco J, Bolondi A, Draghi G, Cetra B, Soni C, Ronderos M, Russo S, Ramírez V, Lager I
Institutos de Virología y Patobiología, CICVyA, INTA-Castelar, CC25, 1712 Castelar, Argentina.
Rev Argent Microbiol. 2002 Jul-Sep;34(3):150-6.
To establish if BTV was circulating in Argentina, 94 bovines from the Santo Tomé and Ituzaingó Departments of Corrientes Province were sampled every 30-60 days during 14 months. Red blood cells from those animals that showed seroconvertion with a c-ELISA were processed for virus isolation by inoculation in embryonated chicken eggs and cell cultures. Cells with CPE were positive by direct and indirect immunofluorescence with BTV specific reagents. These samples examined by electron microscopy showed virus particles with BTV morphological characteristics. Blood samples and tissue culture supernantants were positive by RT-PCR technique with primers corresponding to the segment 3 of the BTV genome. Haematophagous insects were captured in one farm using light traps and Culicoides insignis Lutz was the predominant species detected. This is the first isolation of BTV in Argentina from northeastern bovines without any disease symptom.
为确定蓝舌病毒（BTV）是否在阿根廷传播，在14个月期间，每隔30 - 60天对科连特斯省圣多美和伊图萨因戈县的94头奶牛进行采样。对那些在竞争ELISA（c - ELISA）中出现血清转化的动物的红细胞进行处理，通过接种到鸡胚和细胞培养物中来分离病毒。出现细胞病变效应（CPE）的细胞经BTV特异性试剂进行直接和间接免疫荧光检测呈阳性。通过电子显微镜检查这些样本，显示出具有BTV形态特征的病毒颗粒。血液样本和组织培养上清液经逆转录聚合酶链反应（RT - PCR）技术检测呈阳性，所用引物对应于BTV基因组的第3节段。在一个农场使用诱虫灯捕获吸血昆虫，检测到的主要物种是卢氏库蠓。这是在阿根廷首次从东北部无任何疾病症状的奶牛中分离出BTV。
