Charlier Johannes, Duchateau Luc, Claerebout Edwin, Vercruysse Jozef
Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Prev Vet Med. 2005 May 10;68(2-4):277-88. doi: 10.1016/j.prevetmed.2005.01.011.
An indirect Ostertagia ostertagi ELISA on milk is a promising diagnostic tool in bovine parasitology. Interpretation of the test results requires a good knowledge of the test characteristics. In this study, border effects, the repeatability of the ELISA and the effect of different factors such as storage, skimming and freeze-thaw cycles of the milk samples were investigated. The border effects trial showed that significant border effects can occur. The repeatability trial was conducted over 3 days. An alternative graphical technique to assess the repeatability over a large number of ELISA plates measured over different days was developed. From these graphs, it was obvious that the ODR values obtained on the third day were deviating from the values on the first and second day. On the third day, also abnormal control values were observed. When the control values were normal, 94% of the variability was explained by the milk sample and 6% by assay variability. The expected 95% range of the difference of 2 ODR readings of the same sample on the same plate and the same sample on different plates was -0.14 to 0.14 and -0.16 to 0.16. No extra variability was observed when samples were tested on a different day, however these results are based on the measurement of 2 days. Storage for 2-4 days at 4 degrees C, using whole milk instead of skimmed milk and up to 2 extra freeze-thaw cycles of the milk samples did not significantly affect the test results.
基于牛奶的间接奥斯特他吉线虫ELISA检测是牛寄生虫学中一种很有前景的诊断工具。对检测结果的解读需要对检测特性有充分了解。在本研究中，对边缘效应、ELISA检测的重复性以及不同因素（如牛奶样本的储存、脱脂和冻融循环）的影响进行了研究。边缘效应试验表明可能会出现显著的边缘效应。重复性试验持续了3天。开发了一种替代的图形技术，用于评估在不同日期测量的大量ELISA板上的重复性。从这些图表中可以明显看出，第三天获得的ODR值与第一天和第二天的值存在偏差。在第三天，还观察到了异常的对照值。当对照值正常时,94%的变异性由牛奶样本解释，6%由检测变异性解释。同一样本在同一板上以及不同板上的2个ODR读数差异的预期95%范围分别为-0.14至0.14和-0.16至0.16。当在不同日期对样本进行检测时，未观察到额外的变异性，然而这些结果基于2天的测量。牛奶样本在4摄氏度下储存2至4天、使用全脂牛奶而非脱脂牛奶以及最多额外进行2次冻融循环，均未对检测结果产生显著影响。
