将动力学方法应用于间接酶联免疫吸附测定法，以检测牛奶中奥斯特他线虫抗体。
Applying a kinetic method to an indirect ELISA measuring Ostertagia ostertagi antibodies in milk.
作者信息
Vanderstichel Raphaël, Dohoo Ian, Markham Fred
机构信息
Department of Health Management (Vanderstichel, Dohoo); Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island (Markham).
出版信息
Can J Vet Res. 2015 Jul;79(3):180-3.
Indirect enzyme-linked immunosorbent assays (ELISAs) are frequently run as endpoint ELISAs (e-ELISAs). However, kinetic ELISAs (k-ELISAs) have certain advantages over e-ELISAs. The objective of this study was to understand the relationship between e-ELISA and k-ELISA results. Specifically, to determine whether it was possible to run both k-ELISA and e-ELISA on the same plate and establish an appropriate time interval for k-ELISA measurements. A normalization method for k-ELISA slopes (slope ratio) is proposed. Using an indirect e-ELISA test measuring antibodies against Ostertagia ostertagi in milk from dairy cattle, we found that running a k-ELISA had no effect on optical density ratio results of an e-ELISA on the same plate, and that agreement was very strong at 10, 15, and 28 min, allowing for a reduction in the total processing time for ELISA tests.
间接酶联免疫吸附测定（ELISA）通常作为终点ELISA（e-ELISA）进行。然而，动力学ELISA（k-ELISA）相对于e-ELISA具有某些优势。本研究的目的是了解e-ELISA和k-ELISA结果之间的关系。具体而言，确定是否可以在同一板上同时进行k-ELISA和e-ELISA，并为k-ELISA测量建立合适的时间间隔。提出了一种k-ELISA斜率的标准化方法（斜率比）。使用间接e-ELISA试验检测奶牛牛奶中抗奥斯特他线虫的抗体，我们发现进行k-ELISA对同一板上e-ELISA的光密度比结果没有影响，并且在10、15和28分钟时一致性非常强，从而可以减少ELISA试验的总处理时间。
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