Li Jian-Tong, Lee Pai-Po, Chen Ou-Chuan, Cheng Winton, Kuo Ching-Ming
Department of Aquaculture, National Pingtung University of Science and Technology, Taiwan.
Fish Shellfish Immunol. 2005 Sep;19(3):269-80. doi: 10.1016/j.fsi.2005.01.003.
The total haemocyte count (THC), phenoloxidase activity, respiratory burst (release of superoxide anion), superoxide dismutase activity, and phagocytic activity and clearance efficiency to the pathogen Lactococcus garvieae were measured when freshwater giant prawns Macrobrachium rosenbergii (16.2 +/- 2.1 g) were individually injected with saline, or dopamine at 0.5, 5.0, or 50.0 pmol prawn(-1). The results show that a transient period of immunosuppression occurred between 2 and 8 h after injection of dopamine for all immune parameters except circulating haemocytes and all immune parameters returned to control values within 8-16 h after receiving dopamine. Injection of dopamine also significantly increased the mortality of M. rosenbergii challenged with the pathogen L. garvieae. These results suggest that stress-inducing dopamine suppresses the immune system, which in turn promotes the susceptibility to L. garvieae in M. rosenbergii.
当对体重为（16.2±2.1克）的罗氏沼虾个体分别注射生理盐水、0.5、5.0或50.0皮摩尔/只的多巴胺时，测定了其总血细胞计数（THC）、酚氧化酶活性、呼吸爆发（超氧阴离子释放）、超氧化物歧化酶活性、吞噬活性以及对病原菌格氏乳球菌的清除效率。结果表明，注射多巴胺后2至8小时内，除循环血细胞外的所有免疫参数均出现短暂的免疫抑制期，且在注射多巴胺后8至16小时内所有免疫参数均恢复至对照值。注射多巴胺还显著增加了受到格氏乳球菌攻击的罗氏沼虾的死亡率。这些结果表明，应激诱导的多巴胺会抑制免疫系统，进而增加罗氏沼虾对格氏乳球菌的易感性。
