Choi Jin Young, Park Ji Gweon, Jeong Hyung Bok, Lee Young Don, Takemura Akihiro, Kim Se Jae
Department of Life Science, Cheju National University, 66 Jejudaehakno, Jeju-si, Jeju-do 690-756, South Korea.
Comp Biochem Physiol B Biochem Mol Biol. 2005 May;141(1):49-59. doi: 10.1016/j.cbpc.2005.01.009.
P450 aromatase (P450arom, CYP19), a CYP19 gene product, is a member of the cytochrome P450 superfamily that catalyzes the formation of aromatic C(18) estrogen from C(19) androgen. To begin to understand the molecular mechanisms of P450 aromatase action in the protogynous wrasse, we isolated two cDNAs: one encoding CYP19a from ovary and the other encoding CYP19b from brain. The full-length cDNA of wrasse CYP19a, isolated from ovary cDNA library, is 2020 bp long and encodes 519 amino acids. The amino acid sequence of CYP19a has 62-83% identity with ovary-type aromatases of other teleosts. The full-length cDNA of wrasse CYP19b obtained using 5' and 3' RACE consists of 2666 bp, and its open reading frame encodes 496 amino acids. The deduced amino acid sequence has 62-83% identity with brain-type aromatases of other teleosts. Northern blot analysis identified a single 2.2-kb transcript in the ovary (CYP19a), and a single 2.6-kb transcript in the brain (CYP19b), suggesting that there are single forms of CYP19a and CYP19b, respectively, in the wrasse. RT-PCR assay showed that two CYP19 genes were expressed ubiquitously in various tissues, although each CYP19 subtype was expressed at highest level in the ovary and brain of the wrasse. These results suggest that CYP19 genes act in diverse tissue types, in addition to their effects on the physiological and reproductive functions of estrogen.
细胞色素P450芳香化酶（P450arom，CYP19）是CYP19基因的产物，属于细胞色素P450超家族成员，可催化C19雄激素形成芳香族C18雌激素。为了初步了解雌性先熟隆头鱼中P450芳香化酶作用的分子机制，我们分离出两个cDNA：一个来自卵巢，编码CYP19a；另一个来自脑，编码CYP19b。从卵巢cDNA文库中分离得到的隆头鱼CYP19a全长cDNA为2020 bp，编码519个氨基酸。CYP19a的氨基酸序列与其他硬骨鱼的卵巢型芳香化酶具有62 - 83%的同一性。利用5'和3' RACE获得的隆头鱼CYP19b全长cDNA由2666 bp组成，其开放阅读框编码496个氨基酸。推导的氨基酸序列与其他硬骨鱼的脑型芳香化酶具有62 - 83%的同一性。Northern印迹分析在卵巢中鉴定出一条2.2 kb的转录本（CYP19a），在脑中鉴定出一条2.6 kb的转录本（CYP19b），表明隆头鱼中分别存在单一形式的CYP19a和CYP19b。RT-PCR分析表明，尽管每种CYP19亚型在隆头鱼的卵巢和脑中表达水平最高，但两个CYP19基因在各种组织中均有广泛表达。这些结果表明，CYP19基因除了对雌激素的生理和生殖功能有影响外，还在多种组织类型中发挥作用。
