用于测定血清中维生素B12和叶酸的非同位素结合分析法

Nonisotopic binding assay for measuring vitamin B12 and folate in serum.

作者信息

van der Weide J, Homan H C, Cozijnsen-van Rheenen E, Vivié-Kipp Y, Poortman J, Kraaijenhagen R J

机构信息

Department of Clinical Chemistry and Hematology, Hospital De Lichtenberg, Amersfoort, The Netherlands.

出版信息

Clin Chem. 1992 May;38(5):766-8.

PMID:1582033

Abstract

We evaluated a nonisotopic method (CEDIA; cloned enzyme donor immunoassay) for estimating vitamin B12 and folate in serum. The assays were performed with a Cobas-Mira analyzer. Intra-assay CVs were from 3.7% to 11.0% for vitamin B12 and from 1.2% to 10.7% for folate. Interassay CVs ranged from 9.5% to 11.9% for vitamin B12 and from 6.1% to 18.5% for folate. Linearity was satisfactory, with analytical recovery of 94% at 8.7 and 25.4 nmol/L for folate and 280 and 554 pmol/L for vitamin B12. The detection limit was 3.6 nmol/L for folate and 12.3 pmol/L for vitamin B12. Results of this assay correlated well with those of a conventional radioassay: r = 0.98 for vitamin B12 (n = 51) and r = 0.97 for folate (n = 57). The CEDIA was easy to perform but appeared to be unreliable for use with samples from myeloma patients.

摘要

我们评估了一种用于估算血清中维生素B12和叶酸的非同位素方法（CEDIA；克隆酶供体免疫分析）。检测使用Cobas-Mira分析仪进行。维生素B12的批内变异系数为3.7%至11.0%，叶酸的批内变异系数为1.2%至10.7%。维生素B12的批间变异系数为9.5%至11.9%，叶酸的批间变异系数为6.1%至18.5%。线性良好，叶酸在8.7和25.4 nmol/L时的分析回收率为94%，维生素B12在280和554 pmol/L时的分析回收率为94%。叶酸的检测限为3.6 nmol/L，维生素B12的检测限为12.3 pmol/L。该检测方法的结果与传统放射分析方法的结果相关性良好：维生素B12的r = 0.98（n = 51），叶酸的r = 0.97（n = 57）。CEDIA操作简便，但对于骨髓瘤患者的样本似乎不可靠。