Güven Mustafa, Ozgünen Kerem, Günay Ismail
Cukurova University, Medical School, Department of Biophysics, Adana, Turkey.
Int J Neurosci. 2005 May;115(5):725-34. doi: 10.1080/00207450590523387.
The effects of lidocaine on the action potential of crushed sciatic nerves were investigated. The sciatic nerves of the rats were removed 5, 15, 25, and 38 days after the crushing and the compound action potentials were recorded with sucrose gap technique. The nerves were treated with 1 mM lidocaine and the conduction blocks of nonfrequency dependent block (NFDB) and frequency depended block (FDB) at 10, 40, and 100 Hz were determined. In intact nerves, the NFDB effect of lidocaine was 38.4 +/- 0.7 %. On the 15th day after the crush, the NFDB was increased to 60.1 +/- 1.3%. On the 38th day NFDB was decreased to 46.0 +/- 0.8%. The following days after the crushing, lidocaine caused a very high rate of FDB with 10, 40, and 100 Hz stimulation. The high NFDB ratios approached normal levels with the improvement of regeneration, but FDB ratios continued to stay at high levels. The results showed that the ratios of FDB and NFDB were increased on the crushed nerve. It was concluded that, in the regeneration conditions of crushed rat sciatic nerves, the sensitivity to local anesthetic increases more than in intact nerves.
研究了利多卡因对挤压伤坐骨神经动作电位的影响。在挤压伤后5、15、25和38天取出大鼠的坐骨神经，并用蔗糖间隙技术记录复合动作电位。用1 mM利多卡因处理神经，并测定在10、40和100 Hz时非频率依赖性阻滞（NFDB）和频率依赖性阻滞（FDB）的传导阻滞情况。在完整神经中，利多卡因的NFDB效应为38.4±0.7%。挤压伤后第15天，NFDB增加到60.1±1.3%。在第38天，NFDB降至46.0±0.8%。挤压伤后的接下来几天，利多卡因在10、40和100 Hz刺激下引起非常高的FDB发生率。随着再生的改善，高NFDB比率接近正常水平，但FDB比率持续保持在高水平。结果表明，挤压伤神经上FDB和NFDB的比率增加。得出的结论是，在大鼠挤压伤坐骨神经的再生条件下，对局部麻醉剂的敏感性比完整神经增加得更多。
