乳化异氟烷可逆阻断离体蟾蜍坐骨神经传导。

Reversible conduction block in isolated toad sciatic nerve by emulsified isoflurane.

机构信息

Laboratory of Anesthesia and Critical Care Medicine, State Key Laboratory of Biotherapy of Cancer, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.

出版信息

Anesth Analg. 2010 Apr 1;110(4):1024-9. doi: 10.1213/ANE.0b013e3181d2732f.

DOI:10.1213/ANE.0b013e3181d2732f

PMID:20357146

Abstract

BACKGROUND

Studies have shown that the local use of volatile anesthetics can produce local anesthetic effects. We designed this study to evaluate the characteristics of nerve conduction block of emulsified isoflurane (EI) and compare its nerve blockade with 1%lidocaine, by measuring compound nerve action potential (CNAP) parameters in isolated toad sciatic nerve.

METHODS

One hundred isolated toad sciatic nerves were selected and randomly assigned to 10 groups of 10 each, administered 2% to 8% EI (v/v) (EI(8) group, etc.), 1% lidocaine, 30% Intralipid(R) (Huarui Pharmacy, Wuxi, Jiangsu, China), and Ringer solution (RS) for 10 minutes, respectively. All nerves were then washed and soaked with RS for 10 minutes and 30 minutes. The nerve conduction block effect was represented by CNAP parameters that were recorded by an extracellular recording technique per minute.

RESULTS

The results showed that the negative amplitudes of CNAP were decreased by EI and lidocaine (P < 0.05), and the conduction velocities of CNAP were also decreased at some time points (D7-W3) (P < 0.05). After RS washing, the 2 parameters recovered gradually. The changes in the 2 parameters induced by EI had slower onset rates and faster recoveries than those produced by lidocaine (7 minutes vs 1 minute and 9 minutes vs 30 minutes). The nerve blockade induced by EI was dose dependent (P < 0.05), and the half maximal inhibition concentration of EI was 5.46%.

CONCLUSIONS

EI produced completely reversible and dose-dependent nerve conduction inhibition, which had slower onset and faster recovery compared with those produced by lidocaine.

摘要

背景

研究表明，局部使用挥发性麻醉剂可以产生局部麻醉作用。我们设计了这项研究，以评估乳化异氟醚（EI）的神经传导阻滞特性，并通过测量分离蟾蜍坐骨神经的复合神经动作电位（CNAP）参数来比较其神经阻滞与 1%利多卡因的神经阻滞。

方法

选择 100 根分离的蟾蜍坐骨神经，随机分为 10 组，每组 10 根，分别给予 2%至 8% EI（v/v）（EI（8）组等）、1%利多卡因、30%Intralipid（R）（江苏无锡华瑞制药有限公司）和 Ringer 溶液（RS），持续 10 分钟。然后，所有神经均用 RS 冲洗和浸泡 10 分钟和 30 分钟。通过细胞外记录技术，每分钟记录 CNAP 参数来表示神经传导阻滞效应。

结果

结果表明，EI 和利多卡因均降低了 CNAP 的负振幅（P<0.05），并在某些时间点（D7-W3）降低了 CNAP 的传导速度（P<0.05）。用 RS 冲洗后，2 个参数逐渐恢复。EI 引起的 2 个参数的变化起效较慢，恢复较快，而利多卡因引起的变化则起效较快，恢复较慢（7 分钟比 1 分钟，9 分钟比 30 分钟）。EI 诱导的神经阻滞呈剂量依赖性（P<0.05），EI 的半抑制浓度为 5.46%。