Bulló Mónica, Salas-Salvadó Jordi, García-Lorda Pilar
Unitat de Nutrició Humana, Facultat de Medicina i Ciències de la Salut de Reus, Universitat Rovira i Virgili, Reus, Spain.
Obes Surg. 2005 Mar;15(3):382-6. doi: 10.1381/0960892053576776.
The authors evaluated whether adipose adiponectin expression is related to adipose tissue lipolytic activity and fatty acid oxidation rate in lean and obese women.
The study consisted of 60 adult females distributed in a wide range of adiposity (BMI 24.0-53.4 kg/m2). Body composition was estimated by bioelectrical impedance. Respiratory quotient was measured by open-circuit indirect calorimetry. RT-PCR assays were performed to measure TNFalpha and adiponectin expression in subcutaneous adipose tissue biopsies. Lipolysis studies were performed in fresh tissue samples obtained from subcutaneous abdominal depots during bariatric surgery in 19 morbidly obese females or by an incisional biopsy or during abdominal elective surgery in normal weight and obese females. Glycerol release was measured with a colorimetric endpoint method.
Patients with a higher degree of adiposity showed lower adipose tissue adiponectin expression and hormone-sensitive lipase (HSL) activity than women in the low range of adiposity. HSL activity was positively related to adiponectin expression. No relationship was observed between adiponectin and TNFalpha subcutaneous adipose tissue expression. The positive relationship between respiratory quotient and adiponectin expression was in the limit of statistical significance (P=0.05). Percentage of body fat and mRNA adiponectin explained 26% of the variance of the adipose tissue HSL activity.
Low adipose tissue adiponectin expression observed in obese people may contribute to the progression of obesity and its co-morbidities by modulating hormone-sensitive lipase activity and fatty acid oxidation.
作者评估了肥胖和消瘦女性脂肪组织中脂联素表达是否与脂肪组织脂肪分解活性及脂肪酸氧化率相关。
该研究纳入60名成年女性，她们的肥胖程度差异较大（BMI为24.0 - 53.4kg/m²）。通过生物电阻抗法估算身体成分。采用开路间接量热法测量呼吸商。运用RT-PCR检测皮下脂肪组织活检样本中TNFα和脂联素的表达。对19例病态肥胖女性在减肥手术期间从腹部皮下脂肪库获取的新鲜组织样本，或正常体重及肥胖女性在切开活检或腹部择期手术期间获取的样本进行脂肪分解研究。采用比色终点法测量甘油释放量。
与肥胖程度较低的女性相比，肥胖程度较高的患者脂肪组织脂联素表达及激素敏感性脂肪酶（HSL）活性较低。HSL活性与脂联素表达呈正相关。未观察到脂联素与皮下脂肪组织TNFα表达之间存在关联。呼吸商与脂联素表达之间的正相关关系接近统计学意义（P = 0.05）。体脂百分比和脂联素mRNA解释了脂肪组织HSL活性变异的26%。
肥胖人群中观察到的低脂肪组织脂联素表达可能通过调节激素敏感性脂肪酶活性和脂肪酸氧化，促进肥胖及其合并症的进展。
