Pemberton C L, Whitehead N A, Sebaihia M, Bell K S, Hyman L J, Harris S J, Matlin A J, Robson N D, Birch P R J, Carr J P, Toth I K, Salmond G P C
Mol Plant Microbe Interact. 2005 Apr;18(4):343-53. doi: 10.1094/MPMI-18-0343.
Seven new genes controlled by the quorum-sensing signal molecule N-(3-oxohexanoyl)-L-homoserine lactone (OHHL) have been identified in Erwinia carotovora subsp. carotovora. Using TnphoA as a mutagen, we enriched for mutants defective in proteins that could play a role in the interaction between E. carotovora subsp. carotovora and its plant hosts, and identified NipEcc and its counterpart in E. carotovora subsp. atroseptica. These are members of a growing family of proteins related to Nep1 from Fusarium oxysporum which can induce necrotic responses in a variety of dicotyledonous plants. NipEcc produced necrosis in tobacco, NipEca affected potato stem rot, and both affected virulence in potato tubers. In E. carotovora subsp. carotovora, nip was shown to be subject to weak repression by the LuxR family regulator, EccR, and may be regulated by the negative global regulator RsmA.
在胡萝卜软腐欧文氏菌胡萝卜软腐亚种中，已鉴定出7个受群体感应信号分子N-（3-氧代己酰基）-L-高丝氨酸内酯（OHHL）调控的新基因。我们以TnphoA作为诱变剂，富集那些在胡萝卜软腐欧文氏菌胡萝卜软腐亚种与其植物宿主相互作用中可能发挥作用的蛋白质缺陷型突变体，并鉴定出NipEcc及其在胡萝卜软腐欧文氏菌黑胫亚种中的对应物。它们是与尖孢镰刀菌Nep1相关的一个不断扩大的蛋白质家族的成员，Nep1能在多种双子叶植物中诱导坏死反应。NipEcc在烟草中产生坏死，NipEca影响马铃薯茎腐病，二者均影响马铃薯块茎的毒力。在胡萝卜软腐欧文氏菌胡萝卜软腐亚种中，nip被证明受到LuxR家族调控因子EccR的弱抑制，并且可能受全局性负调控因子RsmA调控。
