Yang Guangyong, Yang Jie, Zhang Qiwei, Wang Wenfeng, Feng Liping, Zhao Li, An Bang, Wang Qiannan, He Chaozu, Luo Hongli
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Corps, Hainan University, Haikou, China.
Hainan Yazhou Bay Seed Laboratory, Sanya Nanfan Research Institute of Hainan University, Sanya, China.
Front Microbiol. 2022 Jun 13;13:911479. doi: 10.3389/fmicb.2022.911479. eCollection 2022.
Fungi secrete numerous effectors to modulate host defense systems. Understanding the molecular mechanisms by which fungal effectors regulate plant defense is of great importance for the development of novel strategies for disease control. In this study, we identified necrosis- and ethylene-inducing protein 1 (Nep1)-like protein (NLP) effector gene, , which contributed to conidial germination, appressorium formation, invasive growth, and virulence of to the rubber tree. Transient expression of CgNLP1 in the leaves of induced ethylene production in plants. Ectopic expression of CgNLP1 in significantly enhanced the resistance to and . An R2R3 type transcription factor HbMYB8-like of rubber tree was identified as the target of CgNLP1.HbMYB8-like, localized on the nucleus, and induced cell death in . CgNLP1 disrupted nuclear accumulation of HbMYB8-like and suppressed HbMYB8-like induced cell death, which is mediated by the salicylic acid (SA) signal pathway. This study suggested a new strategy whereby exploited the CgNLP1 effector to affect invasion and suppress a host defense regulator HbMYB8-like to facilitate infection.
真菌分泌多种效应子来调节宿主防御系统。了解真菌效应子调控植物防御的分子机制对于开发新的病害控制策略至关重要。在本研究中，我们鉴定了坏死和乙烯诱导蛋白1（Nep1）样蛋白（NLP）效应子基因，其有助于分生孢子萌发、附着胞形成、侵入性生长以及对橡胶树的致病性。CgNLP1在烟草叶片中的瞬时表达诱导了植物中乙烯的产生。CgNLP1在拟南芥中的异位表达显著增强了对灰霉病菌和核盘菌的抗性。橡胶树的一个R2R3型转录因子HbMYB8-like被鉴定为CgNLP1的靶标。HbMYB8-like定位于细胞核，并在烟草中诱导细胞死亡。CgNLP1破坏了HbMYB8-like的核积累，并抑制了由水杨酸（SA）信号通路介导的HbMYB8-like诱导的细胞死亡。本研究提出了一种新策略，即灰葡萄孢利用CgNLP1效应子来影响侵染并抑制宿主防御调节因子HbMYB8-like以促进感染。
