Traini Tonino, Degidi Marco, Caputi Sergio, Strocchi Rita, Di Iorio Donato, Piattelli Adriano
Department of Applied Sciences of Oral and Dental Diseases, School of Dentistry, University "G d'Annunzio" of Chieti-Pescara, Italy.
J Periodontol. 2005 Jan;76(1):83-9. doi: 10.1902/jop.2005.76.1.83.
The main factor in determining the mechanical properties of bone is the collagen configuration.
This study investigated the birefringence in human bone around loaded and unloaded titanium dental implants to evaluate the collagen fiber orientation using circularly polarized light (CPL) and scanning electron microscopy (SEM). A total of 10 titanium dental implants, five immediately loaded and five unloaded, were used. The birefringence measurements were performed on digitized images of both loaded and unloaded implants. All images detected at 50x were measured using a software image analysis.
In the bone around loaded implants, the transverse collagen fiber area was 45,481+/-3,037 pixel2 (mean+/-SD), while the area of longitudinal collagen fibers was 13,676+/-2,232 pixel2 (mean+/-SD). In the unloaded implants, the transverse collagen fiber area was 32,174+/-2,554 pixel2 (mean+/-SD), while the area of longitudinal collagen fibers was 89,073+/-1,960 pixel2 (mean+/-SD). The CPL measurements of the birefringence for transverse collagen fibers of loaded versus unloaded implants indicated that the differences were statistically significant (P <0.05). The results for the longitudinal collagen fibers of loaded versus unloaded implants were also statistically significant (P <0.05).
In the bone around loaded dental implants, transverse collagen fibers were more abundant, while in the unloaded implants, collagen fibers run more longitudinally. The load seemed to determine the collagen fiber orientation.
决定骨力学性能的主要因素是胶原蛋白的结构。
本研究通过使用圆偏振光（CPL）和扫描电子显微镜（SEM），研究加载和未加载钛牙种植体周围人骨的双折射，以评估胶原纤维的取向。总共使用了10颗钛牙种植体，其中5颗立即加载，5颗未加载。对加载和未加载种植体的数字化图像进行双折射测量。使用软件图像分析对所有50倍放大倍数下检测到的图像进行测量。
在加载种植体周围的骨中，横向胶原纤维面积为45481±3037像素²（平均值±标准差），而纵向胶原纤维面积为13676±2232像素²（平均值±标准差）。在未加载种植体中，横向胶原纤维面积为32174±2554像素²（平均值±标准差），而纵向胶原纤维面积为89073±1960像素²（平均值±标准差）。加载与未加载种植体横向胶原纤维双折射的CPL测量结果表明差异具有统计学意义（P<0.05）。加载与未加载种植体纵向胶原纤维的结果也具有统计学意义（P<0.05）。
在加载牙种植体周围的骨中，横向胶原纤维更丰富，而在未加载种植体中，胶原纤维更多地纵向排列。负荷似乎决定了胶原纤维的取向。
