Casadevall A, Mukherjee J, Devi S J, Schneerson R, Robbins J B, Scharff M D
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York.
J Infect Dis. 1992 Jun;165(6):1086-93. doi: 10.1093/infdis/165.6.1086.
The antibody responses of BALB/c mice to serotype A Cryptococcus neoformans capsular polysaccharide (CNPS) were compared after cryptococcal infection and immunization with a serotype A glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT). Infection rarely resulted in a rise of serum antibody titer to CNPS. In contrast, mice immunized with GXM-TT produced serum IgM and IgG to CNPS. Six IgM and one IgG1 monoclonal antibodies (MAbs) were generated from the spleen of one infected mouse. Nine IgM, 1 IgG3, 16 IgG1, and 7 IgA MAbs were generated from the spleen of one GXM-TT-immunized mouse. All MAbs generated from both mice bound to the GXM fraction of the capsular polysaccharide. For some MAbs, de-O-acetylation of serotype A GXM abolished or greatly reduced MAb binding compared with the native GXM. All MAbs reacted with CNPS from C. neoformans serotypes A-D. MAbs generated from the infected mouse competitively inhibited the binding of MAbs generated from the GXM-TT-immunized mouse. These results indicate that some antibodies elicited by infection with C. neoformans or by immunization with GXM-TT bind to the same antigenic determinant in the GXM.
在感染新型隐球菌以及用A型葡糖醛酸木聚糖甘露聚糖-破伤风类毒素偶联物（GXM-TT）免疫后，比较了BALB/c小鼠对A型新型隐球菌荚膜多糖（CNPS）的抗体反应。感染很少导致血清抗CNPS抗体滴度升高。相比之下，用GXM-TT免疫的小鼠产生了针对CNPS的血清IgM和IgG。从一只感染小鼠的脾脏中产生了6种IgM和1种IgG1单克隆抗体（MAb）。从一只GXM-TT免疫小鼠的脾脏中产生了9种IgM、1种IgG3、16种IgG1和7种IgA MAb。从两只小鼠产生的所有MAb都与荚膜多糖的GXM部分结合。对于一些MAb，与天然GXM相比，A型GXM的去O-乙酰化消除或大大降低了MAb的结合。所有MAb都与新型隐球菌A-D血清型的CNPS反应。从感染小鼠产生的MAb竞争性抑制了从GXM-TT免疫小鼠产生的MAb的结合。这些结果表明，由新型隐球菌感染或用GXM-TT免疫引发的一些抗体与GXM中的相同抗原决定簇结合。
