Garcia F U, Wojta J, Hoover R L
Pathology Department, Vanderbilt University, Nashville, Tennessee.
J Infect Dis. 1992 Jun;165(6):1138-41. doi: 10.1093/infdis/165.6.1138.
Bartonella bacilliformis, a gram-negative, flagellated, motile bacterium, is the etiologic agent of verruca peruana. It is found within the verruca, where it can form large cytoplasmic (Rocha-Lima) inclusions in endothelial cells. Previously, an activity has been described in homogenates of B. bacilliformis that in vitro increases the proliferation of endothelial cells and their production of tissue-type plasminogen activator (t-PA) and in vivo is angiogenic. The aim of the present study was to determine if live B. bacilliformis similarly stimulated endothelial cells and produced the Rocha-Lima inclusion. By measuring proliferation of cells and the production of t-PA in vitro, it was found that the live bacteria increased both parameters in a fashion similar to the homogenates of B. bacilliformis. Interaction between the bacteria and endothelial cells appeared to be necessary for proliferation. On electron microscopy, bacteria penetrated the endothelial cell within 1 h, forming a small membrane-bound inclusion. By 12 h, a large membrane-bound inclusion, similar to the Rocha-Lima inclusion, containing numerous bacteria was present. These data provide further evidence that B. bacilliformis has an angiogenic activity and that the bacteria are at least in part responsible for the vascular proliferation of the verruca.
杆状巴尔通体是一种革兰氏阴性、具鞭毛、能运动的细菌，是秘鲁疣的病原体。它存在于疣内，可在内皮细胞中形成大的细胞质（罗查-利马）包涵体。此前，已报道杆状巴尔通体匀浆具有一种活性，该活性在体外可增加内皮细胞的增殖及其组织型纤溶酶原激活物（t-PA）的产生，在体内具有血管生成作用。本研究的目的是确定活的杆状巴尔通体是否同样刺激内皮细胞并产生罗查-利马包涵体。通过在体外测量细胞增殖和t-PA的产生，发现活细菌以类似于杆状巴尔通体匀浆的方式增加了这两个参数。细菌与内皮细胞之间的相互作用似乎是增殖所必需的。在电子显微镜下，细菌在1小时内穿透内皮细胞，形成一个小的膜结合包涵体。到12小时时，出现了一个大的膜结合包涵体，类似于罗查-利马包涵体，含有大量细菌。这些数据进一步证明杆状巴尔通体具有血管生成活性，且该细菌至少部分地导致了疣的血管增生。
