Stenfors L E, Räisänen S
Department of Otolaryngology and Clinical Laboratory, Central Hospital of Keski-Pohjanmaa, Kokkola, Finland.
J Infect Dis. 1992 Jun;165(6):1148-50. doi: 10.1093/infdis/165.6.1148.
Attachment of bacteria to nonciliated cells of the nasopharyngeal epithelium was evaluated by immunofluorescence assay in 25 otitis-prone and 25 non-otitis-prone children undergoing ear, nose, or throat surgery under general anesthesia. The bacterial findings were analyzed simultaneously, both qualitatively and quantitatively. In otitis-prone children, there was a significant preponderance of epithelial cells having greater than 50 attached bacteria (P less than .001) and of epithelial cells with attached Streptococcus pneumoniae and Haemophilus influenzae (P less than .05). Also, the occurrence of Branhamella catarrhalis in the nasopharynx was more pronounced in the otitis-prone group (P less than .05). No significant differences in the occurrence of other middle ear pathogens or quantitative dominance of pathogens were noted between the two groups. Abundant attachment of pathogens to the epithelial cells close to the nasopharyngeal orifice of the eustachian tube is of a significant factor for the development of the otitis-prone condition.
通过免疫荧光测定法，对25名易患中耳炎和25名不易患中耳炎的儿童进行评估，这些儿童均在全身麻醉下接受耳鼻喉手术。同时对细菌检测结果进行了定性和定量分析。在易患中耳炎的儿童中，附着细菌超过50个的上皮细胞（P<0.001）以及附着有肺炎链球菌和流感嗜血杆菌的上皮细胞（P<0.05）明显占优势。此外，卡他莫拉菌在易患中耳炎组的鼻咽部出现更为明显（P<0.05）。两组之间在其他中耳病原体的出现或病原体的数量优势方面未发现显著差异。病原体大量附着于咽鼓管鼻咽口附近的上皮细胞是易患中耳炎状况发展的一个重要因素。
