Mannelli Ilaria, Minunni Maria, Tombelli Sara, Wang Ronghui, Michela Spiriti Maria, Mascini Marco
Dipartimento di Chimica, Università degli Studi di Firenze, Polo Scientifico, Via della Lastruccia 3, Sesto Fiorentino 50019, Italy.
Bioelectrochemistry. 2005 Apr;66(1-2):129-38. doi: 10.1016/j.bioelechem.2004.04.008.
An immobilisation procedure based on the direct coupling of thiolated probes (Probe-C6-SH) to bare gold sensor surfaces has been compared with a reference immobilisation method, based on the coupling of biotinylated probes onto a streptavidin-coated dextran-modified surface. The instrumentations used were a quartz crystal microbalance (QCM) and the optical instruments Biacore X and Spreeta based on surface plasmon resonance (SPR). The performances of the DNA-based sensors resulting from direct coupling of thiolated DNA probes onto electrodes of quartz crystals or gold SPR-chips have been studied in terms of the main analytical parameters, i.e. selectivity, sensitivity, reproducibility, etc. In particular, the two immobilisation approaches have been applied to the analysis of oligonucleotides, DNA amplified by polymerase chain reaction (PCR) and genomic DNA enzymatically digested.
一种基于硫醇化探针（Probe-C6-SH）直接偶联到裸金传感器表面的固定化方法，已与一种参考固定化方法进行了比较，后者是基于将生物素化探针偶联到链霉亲和素包被的葡聚糖修饰表面。所使用的仪器包括石英晶体微天平（QCM）以及基于表面等离子体共振（SPR）的光学仪器Biacore X和Spreeta。已根据主要分析参数，即选择性、灵敏度、重现性等，研究了将硫醇化DNA探针直接偶联到石英晶体电极或金SPR芯片上所得到的基于DNA的传感器的性能。特别是，这两种固定化方法已应用于寡核苷酸、聚合酶链反应（PCR）扩增的DNA以及酶切基因组DNA的分析。
