Wang D, An S-H, Guo Z-J, Xu H-J, Zhang C-X
Institute of Applied Entomology, Zhejiang University, Hangzhou, P.R. China.
Arch Virol. 2005 Aug;150(8):1505-15. doi: 10.1007/s00705-005-0534-9. Epub 2005 Apr 18.
Homologues of Helicoverpa armigera nucleopolyhedrovirus (HearSNPV) orf33 are found in all 22 completely sequenced members of the lepidopteran nucleopolyhedroviruses and granuloviruses, but so far their functions are unknown. In this report, we describe the characterization of HearSNPV orf33 (ha33). Northern blot analysis showed a single transcript of ha33 of approximately 0.7 kb was transcribed beginning at 3 h post-infection in infected Helicoverpa zea cells (HzAM1) and the gene product could be detected as early as 6 h post-infection by western blot analysis using a rabbit derived polyclonal antibody, suggesting it was an early gene. Western blot analysis also demonstrated the ha33 protein in infected cells was a 31 kDa protein, larger than the theoretical size of 28.4 kDa, and located in the envelope fraction of budded virions (BVs). The results suggested that HearSNPV ha33 gene is a functional gene that encodes a novel structural protein of baculovirus BVs, BV-e31.
在鳞翅目核型多角体病毒和颗粒体病毒的所有22个全序列成员中均发现了棉铃虫核型多角体病毒（HearSNPV）orf33的同源物，但迄今为止其功能尚不清楚。在本报告中，我们描述了HearSNPV orf33（ha33）的特征。Northern印迹分析显示，感染的玉米螟细胞（HzAM1）在感染后3小时开始转录出一条约0.7 kb的ha33单转录本，并且通过使用兔源多克隆抗体的Western印迹分析，早在感染后6小时就能检测到该基因产物，表明它是一个早期基因。Western印迹分析还表明，感染细胞中的ha33蛋白是一种31 kDa的蛋白，大于理论大小28.4 kDa，并且位于出芽病毒粒子（BVs）的包膜部分。结果表明，HearSNPV ha33基因是一个功能基因，编码杆状病毒BVs的一种新型结构蛋白BV-e31。
