Tian C-H, Tang X-D, Xu H-J, Ge J-Q, Miao Y-G, Zhang C-X
Institute of Insect Science, Zhejiang University, Kaixuan Road 268, Hangzhou, 310029, People's Republic of China.
Virus Genes. 2009 Feb;38(1):171-7. doi: 10.1007/s11262-008-0312-3. Epub 2008 Dec 16.
Bombyx mori nucleopolyhedrovirus (BmNPV) ORF 51 (Bm51) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm51 was characterized. Transcripts of Bm51 were detected from 4.5 through 72 hour post infection (h p.i.) by RT-PCR. The corresponding protein was detected from 6 to 72 h p.i. in BmNPV-infected BmN cells by western blot analysis using a polyclonal antibody against Bm51. Western blot assay of occlusion-derived virus and budded virus (BV) preparations revealed that Bm51 encodes a 23-kDa structural protein that is associated with BV and is located in the envelope fraction of budded virions. The protein was temporarily called BV-E23. In addition immunofluorescence microscopy demonstrated that the protein was present within the cytoplasm and nuclei in virus-infected cells. In conclusion, the available data suggest that Bm51 is a functional ORF of BmNPV and encodes a protein expressed in the early stage of the infection cycle that is associated with the BV envelope.
家蚕核型多角体病毒（BmNPV）的开放阅读框51（Bm51）是许多鳞翅目NPV中存在的一个基因，但其功能尚不清楚。在本研究中，对Bm51进行了特性分析。通过RT-PCR在感染后4.5小时至72小时（h p.i.）检测到Bm51的转录本。使用针对Bm51的多克隆抗体，通过蛋白质免疫印迹分析在BmNPV感染的BmN细胞中于感染后6至72小时检测到相应的蛋白质。对多角体衍生病毒和出芽病毒（BV）制剂的蛋白质免疫印迹分析表明，Bm51编码一种23 kDa的结构蛋白，该蛋白与BV相关，位于出芽病毒粒子的包膜部分。该蛋白暂时称为BV-E23。此外，免疫荧光显微镜显示该蛋白存在于病毒感染细胞的细胞质和细胞核内。总之，现有数据表明Bm51是BmNPV的一个功能性开放阅读框，编码一种在感染周期早期表达的与BV包膜相关的蛋白质。
