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Denaturation of metalloproteins with EDTA to facilitate enzymatic digestion and mass fingerprinting.

作者信息

Janecki Dariusz J, Reilly James P

机构信息

Chemistry Department, Indiana University, Bloomington, 47405, USA.

出版信息

Rapid Commun Mass Spectrom. 2005;19(10):1268-72. doi: 10.1002/rcm.1924.

DOI:10.1002/rcm.1924
PMID:15834845
Abstract

Metal ions bound to a protein often stabilize tertiary and/or quaternary structure. Consequently, the digestion of metalloproteins that precedes analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is frequently incomplete. It is demonstrated that ethylenediaminetetraacetic acid (EDTA) successfully destabilizes metalloprotein structure and thereby facilitates tryptic digestion and protein identification.

摘要

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