Xu Songyun, Pan Chensong, Hu Ligang, Zhang Yu, Guo Zhong, Li Xin, Zou Hanfa
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Electrophoresis. 2004 Nov;25(21-22):3669-76. doi: 10.1002/elps.200406063.
Desorption/ionization on silicon mass spectrometry (DIOS-MS) is a matrix-free technique that allows for the direct desorption/ionization of low-molecular-weight compounds with little or no fragmentation of analytes. This technique has a relatively high tolerance for contaminants commonly found in biological samples. DIOS-MS has been applied to determine the activity of immobilized enzymes on the porous silicon surface. Enzyme activities were also monitored with the addition of a competitive inhibitor in the substrate solution. It is demonstrated that this method can be applied to the screening of enzyme inhibitors. Furthermore, a method for peptide mapping analysis by in situ digestion of proteins on the porous silicon surface modified by trypsin, combined with matrix-assisted laser desorption/ionization-time of flight-MS has been developed.
硅基解吸/电离质谱法（DIOS-MS）是一种无基质技术，可实现低分子量化合物的直接解吸/电离，且分析物很少或几乎不发生碎片化。该技术对生物样品中常见的污染物具有较高的耐受性。DIOS-MS已用于测定多孔硅表面固定化酶的活性。还通过在底物溶液中添加竞争性抑制剂来监测酶活性。结果表明，该方法可用于酶抑制剂的筛选。此外，还开发了一种通过胰蛋白酶修饰的多孔硅表面上蛋白质的原位消化，结合基质辅助激光解吸/电离飞行时间质谱进行肽图谱分析的方法。
