结缔组织生长因子反义寡核苷酸对体外培养的人瘢痕疙瘩成纤维细胞的影响
[Effects of connective tissue growth factor antisense oligonucleotides on the cultured human keloid fibroblasts in vitro].
作者信息
Liu Jian-yi, Li Shi-rong, Ji Shu-xing
机构信息
The Department of Plastic and Aesthetic Surgery, the Southwest Hospital of the Third Military Medical University, Chongqing 400038, China.
出版信息
Zhonghua Zheng Xing Wai Ke Za Zhi. 2004 Nov;20(6):454-6.
OBJECTIVE
To explore the effects of connective tissue growth factor (CTGF) on the pathogenesis of human keloid.
METHODS
CTGF antisense oligonucleotides (ASODN) conjugated with isothiocyanate fluorescence was encapsulated by liposome, and then added into the human keloid fibroblast (HKF) culturing media. The intracellular distribution of CTGF ASODN was observed with fluorescence microscopy in the fixed HKF. The proliferation of HKF was measured by MIT test. The apoptosis of HKF was measured with a flow cytometer. The collagen synthesis of HKF was measured by using H3-proline incorporation method.
RESULTS
The CTGF ASODN inhibited the proliferation and collagen synthesis of the HKF, compared with the control, but it increased the apoptosis after the transfection (P < 0.01).
CONCLUSION
CTGF ASODN may has anti-fibrotic effects on human keloid in vitro, and the CTGF may play an important role in promoting the fibrosis of human keloid.
目的
探讨结缔组织生长因子（CTGF）在人瘢痕疙瘩发病机制中的作用。
方法
将异硫氰酸荧光素偶联的CTGF反义寡核苷酸（ASODN）用脂质体包裹，然后加入人瘢痕疙瘩成纤维细胞（HKF）培养基中。在固定的HKF中用荧光显微镜观察CTGF ASODN的细胞内分布。用MTT法检测HKF的增殖。用流式细胞仪检测HKF的凋亡。用H3-脯氨酸掺入法检测HKF的胶原合成。
结果
与对照组相比，CTGF ASODN抑制了HKF的增殖和胶原合成，但转染后增加了凋亡（P<0.01）。
结论
CTGF ASODN在体外可能对人瘢痕疙瘩具有抗纤维化作用，且CTGF可能在促进人瘢痕疙瘩纤维化中起重要作用。
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