Zhu Rongyi, Yue Baishuang, Yang Qiaorong, Ma Ying, Huang Guiqin, Guan Ming, Avram Mathew M, Lu Zhong
Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, 200040, China; Department of Dermatology, Huadong Hospital, Fudan University, Shanghai, 200040, China.
Lasers Surg Med. 2015 Feb;47(2):203-9. doi: 10.1002/lsm.22334.
To investigate the effect of pulsed dye laser (PDL 595 nm) on the proliferation and expression of connective tissue growth factor (CTGF) in cultured keloid fibroblasts.
Cultured keloid fibroblasts were exposed to pulsed dye laser irradiation at fluences of 6, 8, and 10 J/cm(2) , with pulse durations of 1.5, 3, and 10 ms. The viability of keloid fibroblasts was measured with CCK-8 at 72, 24, and 12 hours prior to irradiation. Subsequently, viability was measured at 12, 24, and 72 hours post-irradiation. Additionally, the fibroblast cell cycle and apoptosis rate were measured by flow cytometry. Finally, keloid fibroblasts underwent real-time polymerase chain reaction (PCR) and Western blot to investigate the CTGF mRNA and protein expression after PDL irradiation. The untreated cultured keloid fibroblasts served as controls.
The proliferation of keloid fibroblasts was significantly inhibited after PDL irradiation. Both CTGF mRNA and protein expression were significantly down-regulated in 1.5, 3, and 10 ms pulse duration groups, in a dose dependent manner (P < 0.05). However, there was no statistically significant difference between groups of different pulse duration in 6, 8, and 10 J/cm(2) fluence ranges (P > 0.05).
Within certain fluence ranges, pulsed dye laser can effectively suppress the growth of keloids and significantly down-regulate CTGF mRNA and CTGF expression.
探讨脉冲染料激光（595nm）对培养的瘢痕疙瘩成纤维细胞增殖及结缔组织生长因子（CTGF）表达的影响。
将培养的瘢痕疙瘩成纤维细胞分别用6、8和10J/cm²的能量密度，1.5、3和10ms的脉冲持续时间进行脉冲染料激光照射。在照射前72、24和12小时用CCK-8检测瘢痕疙瘩成纤维细胞的活力。随后，在照射后12、24和72小时检测活力。此外，通过流式细胞术检测成纤维细胞的细胞周期和凋亡率。最后，对瘢痕疙瘩成纤维细胞进行实时聚合酶链反应（PCR）和蛋白质印迹法，以研究脉冲染料激光照射后CTGF mRNA和蛋白质的表达。未处理的培养瘢痕疙瘩成纤维细胞作为对照。
脉冲染料激光照射后瘢痕疙瘩成纤维细胞的增殖明显受到抑制。在1.5、3和10ms脉冲持续时间组中，CTGF mRNA和蛋白质表达均呈剂量依赖性显著下调（P<0.05）。然而，在6、8和10J/cm²能量密度范围内，不同脉冲持续时间组之间无统计学显著差异（P>0.05）。
在一定能量密度范围内，脉冲染料激光可有效抑制瘢痕疙瘩的生长，并显著下调CTGF mRNA和CTGF表达。
