[分子量为32000的抗原在广州管圆线虫免疫诊断中的价值]
[Value of the antigen with molecular mass of 32 000 in immunodiagnosis of Angiostrongylus cantonensis].
作者信息
Li Hua, Chen Xiao-Guang, Shen Hao-Xian, Chen Dai-Xiong, Qiu Yu-Rong, Hu Xiao-Jia
机构信息
Department of Parasitology, Southern Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2005 Apr;25(4):380-3.
OBJECTIVE
To evaluate the specificity and sensitivity of immunodiagnosis with the antigen with molecular mass of 32 000 of Angiostrongyliasis cantonensis(AC32).
METHODS
The major antigenic protein AC32 was purified from the antigens of A.cantonensis adult worm by electroelution from SDS-polyacrylamide gels. AC32-enzyme linked immunosorbent assay (ELISA) and adult worm antigen (AWA)-ELISA were used to detect the specific IgG in the sera of normal rats (n=5) and rats with angiostrongyliasis (n=61), sera of healthy individuals (n=50) and patients (n=50) with angiostrongyliasis, schistosomiasis and other parasitosis.
RESULTS
The positivity rate in AC32-ELISA of the sera from rats with angiostrongyliasis was 100%; without false positive results or cross reaction between AC32 and the sera of the normal control and patients infected with parasites other than A.cantonensis.
CONCLUSION
AC32 of A.cantonensis is a valuable candidate antigen for immunodiagnosis of angiostrongyliasis with high sensitivity and specificity.
目的
评估广州管圆线虫分子量为32000的抗原（AC32）免疫诊断的特异性和敏感性。
方法
通过SDS-聚丙烯酰胺凝胶电洗脱法从广州管圆线虫成虫抗原中纯化主要抗原蛋白AC32。采用AC32-酶联免疫吸附测定法（ELISA）和成虫抗原（AWA）-ELISA检测正常大鼠（n = 5）和广州管圆线虫病大鼠（n = 61）、健康个体（n = 50）以及广州管圆线虫病、血吸虫病和其他寄生虫病患者（n = 50）血清中的特异性IgG。
结果
广州管圆线虫病大鼠血清AC32-ELISA阳性率为100%；AC32与正常对照及感染除广州管圆线虫外其他寄生虫的患者血清之间无假阳性结果或交叉反应。
结论
广州管圆线虫AC32是一种有价值的候选抗原，用于广州管圆线虫病的免疫诊断，具有高敏感性和特异性。
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