Nuamtanong S
Department of Parasitology, School of Medicine, Hiroshima University, Japan.
Southeast Asian J Trop Med Public Health. 1996 Jun;27(2):291-6.
The objective of this study was to evaluate the crude somatic extract of female Angiostrongylus cantonensis by ELISA and to evaluate the 29 kDa and the 31 kDa components of female worm by EITB for serodiagnosis of human angiostrongyliasis. Using ELISA, cross reaction occurred among all sera tested except capillariasis. The sensitivity, specificity, positive and negative predictive values of ELISA were found to be 100%, 66.8%, 27.1% and 100% respectively. Using EITB, The 29 kDa component cross-reacted with most of the heterologous sera while the 31 kDa component was recognized by 69.2% (18/26) sera from angiostrongyliasis patients, cross-reacted only with trichinellosis, trichuriasis and opisthorchiasis sera. This component may possibly used as reliable agents for angiostrongyliasis out of other tissue infected helminthiasis. The sensitivity, specificity, positive and negative predictive values of EITB for the 31.0 kDa component were 69.2%, 82.4%, 46.2% and 92.5% respectively.
本研究的目的是通过酶联免疫吸附测定（ELISA）评估广州管圆线虫雌虫的粗体壁提取物，并通过免疫印迹法（EITB）评估雌虫的29 kDa和31 kDa组分，用于人体管圆线虫病的血清学诊断。使用ELISA检测时，除毛细线虫病外，所有检测血清之间均发生交叉反应。ELISA的敏感性、特异性、阳性预测值和阴性预测值分别为100%、66.8%、27.1%和100%。使用EITB检测时，29 kDa组分与大多数异源血清发生交叉反应，而31 kDa组分被69.2%（18/26）的管圆线虫病患者血清识别，仅与旋毛虫病血清、鞭虫病血清和华支睾吸虫病血清发生交叉反应。该组分可能作为一种可靠的试剂，用于区分管圆线虫病与其他组织感染的蠕虫病。EITB对31.0 kDa组分的敏感性、特异性、阳性预测值和阴性预测值分别为69.2%、82.4%、46.2%和92.5%。
